| Quantum Dot Targeting with Lipoic Acid Ligase and HaloTag for Single Molecule Imaging on Living Cells. | |
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MedLine Citation:
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PMID: 23181687 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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We present a methodology for targeting quantum dots to proteins on living cells in two steps. In the first step, E. coli lipoic acid ligase (LplA) site-specifically attaches 10-bromodecanoic acid onto a 13-amino acid peptide that can be genetically fused to a protein of interest. In the second step, quantum dots derivatized with HaloTag, a modified haloalkane dehalogenase, react with the ligated bromodecanoic acid to form a covalent adduct. We found this targeting method to be specific, fast, and fully orthogonal to a previously reported and analogous quantum dot targeting method using E. coli biotin ligase and streptavidin. We used these two methods in combination for two-color quantum dot visualization of different proteins expressed on the same cell or on neighboring cells. Both methods were also used to track single molecules of neurexin, a synaptic adhesion protein, to measure its lateral diffusion in the presence of neuroligin, its trans-synaptic adhesion partner. |
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Authors:
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Daniel S Liu; William S Phipps; Ken H Loh; Mark Howarth; Alice Y Ting |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-11-26 |
Journal Detail:
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Title: ACS nano Volume: - ISSN: 1936-086X ISO Abbreviation: ACS Nano Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-11-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101313589 Medline TA: ACS Nano Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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