Document Detail

Quantity and conformation of lysozyme deposited on conventional and silicone hydrogel contact lens materials using an in vitro model.
MedLine Citation:
PMID:  17502748     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To determine the activity of hen egg lysozyme (HEL) deposited on conventional and silicone hydrogel contact lens materials by using an in vitro model. METHODS: ACUVUE 2 (etafilcon A), PureVision (balafilcon A), ACUVUE Advance (galyfilcon A), Focus NIGHT & DAY (lotrafilcon A), O2 Optix (lotrafilcon B), Proclear (omafilcon A), and ACUVUE OASYS (senofilcon A) contact lenses were deposited in vitro in a phosphate-buffered solution (PBS) containing 2 mg/mL HEL. Lenses were briefly rinsed in PBS to remove unbound material and extracted in a mixture of acetonitrile and trifluoroacetic acid. After lyophilization, extracts were examined for lysozyme activity by micrococcal assay and total protein by Western blot. RESULTS: In terms of total protein accumulation, ACUVUE 2 showed the most, with 1,800 microg per lens. Proclear was next, with 68 microg per lens, and Focus NIGHT & DAY showed the least, with 2 microg per lens. ACUVUE Advance, ACUVUE OASYS, and O2 Optix accumulated similar amounts of lysozyme, at approximately 6 microg per lens. Lysozyme deposited on ACUVUE 2 showed the greatest activity (91% +/- 5%), and this result was statistically different from all other lens types (P<0.001). Lysozyme deposited on Focus NIGHT & DAY (24% +/- 5%) and O2 Optix (23% +/- 11%) showed the lowest activity. Lysozyme deposits on other lens materials showed intermediate activity (ACUVUE Advance, 60% +/- 15%; ACUVUE OASYS, 51% +/- 9%; PureVision, 58% +/- 8%; and Proclear, 38% +/- 3%). CONCLUSIONS: Silicone hydrogel lenses acquire less lysozyme deposit than conventional group II (Proclear) or group IV (ACUVUE 2) lenses do, and the levels of activity of the lysozyme are highly variable between materials.
Maciej Suwala; Mary-Ann Glasier; Lakshman N Subbaraman; Lyndon Jones
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Eye & contact lens     Volume:  33     ISSN:  1542-2321     ISO Abbreviation:  Eye Contact Lens     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-05-15     Completed Date:  2007-10-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101160941     Medline TA:  Eye Contact Lens     Country:  United States    
Other Details:
Languages:  eng     Pagination:  138-43     Citation Subset:  IM    
Centre for Contact Lens Research, School of Optometry, University of Waterloo, Waterloo, Ontario, Canada.
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MeSH Terms
Contact Lenses* / classification
Muramidase / metabolism*,  pharmacokinetics*
Ovum / enzymology
Reg. No./Substance:
0/Silicones; 25852-47-5/Hydrogel; EC

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