Document Detail


Quantitative ultrasound assessment of the rat cervix.
MedLine Citation:
PMID:  16870896     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: The purpose of this research was to detect cervical ripening with a new quantitative ultrasound technique.
METHODS: Cervices of 13 nonpregnant and 65 timed pregnant (days 15, 17, 19, 20, and 21 of pregnancy) Sprague Dawley rats were scanned ex vivo with a 70-MHz ultrasound transducer. Ultrasound scatterer property estimates (scatterer diameter [SD], acoustic concentration [AC], and scatterer strength factor [SSF]) from the cervices were quantified and then compared to hydroxyproline and water content. Insertion loss (attenuation) was measured in 3 rats in each of the 6 groups. Discriminant analysis was used to predict gestational age group (cervical ripening) from the ultrasound variables SD, SSF, and AC.
RESULTS: Differences were observed between the groups (SD, AC, and SSF; P < .0001). Quantitative ultrasound measures changed as the cervix ripened: (1) SD increased from days 15 to 21; (2) AC decreased from days 15 to 21; and (3) SSF was the greatest in the nonpregnant group and the least in the day 21 group. Cervix hydroxyproline content increased as pregnancy progressed (P < .003) and correlated with group, SD, AC, and SSF (P < .001). Discriminant analysis of ultrasound variables predicted 56.4% of gestational group assignment (P < .001) and increased to 77% within 2 days of the predicted analysis. Cervix insertion loss was greatest for the nonpregnant group and least for the day 21 group.
CONCLUSIONS: Quantitative ultrasound predicted cervical ripening in the rat cervix, but before use in humans, quantitative ultrasound will need to predict gestational age in the later days of gestation with more precision.
Authors:
Barbara L McFarlin; William D O'Brien; Michael L Oelze; James F Zachary; Rosemary C White-Traut
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine     Volume:  25     ISSN:  0278-4297     ISO Abbreviation:  J Ultrasound Med     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-07-27     Completed Date:  2006-12-07     Revised Date:  2011-01-24    
Medline Journal Info:
Nlm Unique ID:  8211547     Medline TA:  J Ultrasound Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1031-40     Citation Subset:  IM    
Affiliation:
Department of Maternal Child Nursing, University of Illinois at Chicago, IL 60612, USA. bmcfar1@uic.edu
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animals
Cervical Ripening*
Cervix Uteri / metabolism,  ultrasonography*
Discriminant Analysis
Female
Hydroxyproline / metabolism
Nitrogen / metabolism
Pregnancy
Prospective Studies
Rats
Rats, Sprague-Dawley
Grant Support
ID/Acronym/Agency:
F31 NR008191-01A1/NR/NINR NIH HHS; F31 NR08191-01A1/NR/NINR NIH HHS
Chemical
Reg. No./Substance:
51-35-4/Hydroxyproline; 7727-37-9/Nitrogen
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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