Document Detail


Quantitative three-dimensional structural analysis of Exophiala dermatitidis yeast cells by freeze-substitution and serial ultrathin sectioning.
MedLine Citation:
PMID:  12868584     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The morphologies, numbers, sizes and volumes of all organelles and cell components identified on ultrathin sections of aerobically grown exponential phase yeast cells of Exophiala dermatitidis in G1 phase were examined by freeze-substitution fixation and serial ultrathin sectioning. The cell wall consisted of three layers and occupied approximately 22% of the cell volume. The nucleus was approximately 1.8 microm in diameter and occupied approximately 7% of the cell volume. There was only one nucleolus in the nucleus and it occupied approximately 16% of the nuclear volume. There were 17-52 mitochondria per cell, occupying 7-12% of the cell volume. Five to ten endoplasmic reticula were present per cell; these occupied only 0.2% of the cell volume and did not form a network. There were 1-4 vacuoles per cell and they occupied 4-10% of the cell volume. Storage material was round and electron transparent and occupied 4-11% of the cell volume. The cytosol occupied 43-53% of the cell volume. The Golgi apparatus, spindle pole body, autophagosomes, multivesicular bodies, lipid bodies, microtubules and microfilaments occupied approximately 1% of the cell volume in total. About 200,000 ribosome particles, 1000 glycogen granules and several tens of microtubules (average length 0.78 microm) were present per yeast cell. The membranes of this yeast could be classified into three groups by their appearance and thickness. This is the first report, to our knowledge, that analysed all the components in the yeast cell quantitatively and in three dimensions, and provides fundamental information for understanding various aspects of cell biology.
Authors:
Sondip Kumar Biswas; Masashi Yamaguchi; Norihide Naoe; Teruhiro Takashima; Kanji Takeo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of electron microscopy     Volume:  52     ISSN:  0022-0744     ISO Abbreviation:  J Electron Microsc (Tokyo)     Publication Date:  2003  
Date Detail:
Created Date:  2003-07-18     Completed Date:  2003-09-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7611157     Medline TA:  J Electron Microsc (Tokyo)     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  133-43     Citation Subset:  IM    
Affiliation:
Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan.
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MeSH Terms
Descriptor/Qualifier:
Cell Nucleus / ultrastructure
Cell Wall / ultrastructure
Exophiala / cytology,  ultrastructure*
Freeze Substitution
Humans
Image Processing, Computer-Assisted / methods*
Imaging, Three-Dimensional / methods*
Microscopy, Electron / methods
Microtomy / methods
Organelles / ultrastructure
Particle Size

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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