| Quantitative three-dimensional imaging of live avian embryonic morphogenesis via micro-computed tomography. | |
| | |
MedLine Citation:
|
PMID: 21761480 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Many clinically relevant congenital malformations arise during mid to late embryonic stages. This period is challenging to image quantitatively in live embryos, necessitating the use of multiple specimens with increased experimental variability. Here we establish X-ray and blood-pool computed tomography (CT) contrast agent toxicity and teratogenesis thresholds for 3D Micro-CT imaging of live avian embryos. Day 4 chick embryos micro-injected with Visipaque™ (VP) developed for an additional 6 days without defect. X-ray radiation up to 798 mGy was nontoxic. Peak average contrast of 1,060 HU occurred within 1 hr of imaging at 50 μm resolution. VP-enhanced contrast persisted past 24 hr with delayed accumulation in the allantois. Regional volumes of VP-injected embryos were statistically identical to those of fixed embryos perfused with osmium tetroxide. We further quantified longitudinal volumetric morphogenesis of the allantois over 30 hr. These results demonstrate the safety and efficacy of contrast enhanced quantitative micro-CT imaging for live embryos. Developmental Dynamics 240:1949-1957, 2011. © 2011 Wiley-Liss, Inc. |
| | |
Authors:
|
Alyssa L Henning; Michael X Jiang; Huseyin C Yalcin; Jonathan T Butcher |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Developmental dynamics : an official publication of the American Association of Anatomists Volume: 240 ISSN: 1097-0177 ISO Abbreviation: Dev. Dyn. Publication Date: 2011 Aug |
Date Detail:
|
Created Date: 2011-07-15 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9201927 Medline TA: Dev Dyn Country: United States |
Other Details:
|
Languages: eng Pagination: 1949-57 Citation Subset: IM |
Copyright Information:
|
Copyright © 2011 Wiley-Liss, Inc. |
Affiliation:
|
Department of Biomedical Engineering, Cornell University, Ithaca, New York. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: PCP effector proteins inturned and fuzzy play nonredundant roles in the patterning but not convergen...
Next Document: Developmental expression of the fermitin/kindlin gene family in Xenopus laevis embryos.