| Quantitative telomerase activity in circulating human leukocytes: utility of real-time telomeric repeats amplification protocol (RQ-TRAP) in a clinical/epidemiological setting. | |
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MedLine Citation:
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PMID: 19496734 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: There is accumulating evidence from the epidemiological field of telomere biology that telomere length plays an important role in the pathophysiology of cardiovascular disease. The RNA-dependent DNA polymerase, telomerase, is essential in regulating telomere length by acting as a reverse transcriptase. However, the relationship between telomerase activity and telomere length in cardiovascular disease is unclear. This is due, in part, to the paucity of information on the utility of a quantitative and routine assay for the determination of telomerase activity in circulating blood leukocytes. METHODS: We used a validated, high-sensitive real-time quantitative telomeric repeat amplification protocol (RQ-TRAP) to determine telomerase activity in circulating blood leukocytes. RESULTS: The present investigation demonstrated direct and reliable detection of telomerase activity of circulating blood leukocytes. CONCLUSION: The present investigation suggests the feasibility of using RQ-TRAP assay in routine screening of telomerase activity in blood specimens typically collected in a clinical/epidemiological setting. |
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Authors:
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Vasco Lança; Robert Y L Zee; Alicia Rivera; Jose R Romero |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical chemistry and laboratory medicine : CCLM / FESCC Volume: 47 ISSN: 1434-6621 ISO Abbreviation: Clin. Chem. Lab. Med. Publication Date: 2009 |
Date Detail:
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Created Date: 2009-07-06 Completed Date: 2009-11-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9806306 Medline TA: Clin Chem Lab Med Country: Germany |
Other Details:
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Languages: eng Pagination: 870-3 Citation Subset: IM |
Affiliation:
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Division of Endocrinology, Diabetes and Hypertension, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Cardiovascular Diseases / genetics Cell Count Epidemiologic Studies Humans Leukocytes / enzymology* Male Nucleic Acid Amplification Techniques* Reagent Kits, Diagnostic Repetitive Sequences, Nucleic Acid Telomerase / analysis* Telomere / chemistry |
| Grant Support | |
ID/Acronym/Agency:
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DK069388/DK/NIDDK NIH HHS; ES014462/ES/NIEHS NIH HHS; HL090632-A1/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Reagent Kits, Diagnostic; EC 2.7.7.49/Telomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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