Document Detail


Quantitative study of soybean-induced changes in proliferation and programmed cell death in the intestinal mucosa of young rats.
MedLine Citation:
PMID:  17228100     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The use of soybean in human and animal nutrition is limited because of high content of bioactive compounds: enzyme inhibitors, polyphenols, goitrogens, phytates, saponins, sugars, and agglutinins. The damage of intestinal mucosa structure was previously observed in animals fed soybean supplemented diets. Hence, the objectives of the presented study were to compare intensity of epithelium remodeling processes in different intestinal segments, and to evaluate the influence of the 1% of soybean dietary supplementation on the processes in intestinal mucosa. The experiment was performed on 30 Wistar rats fed AIN-93 based diets. Animals were divided randomly into three groups: control (CTRL), with 1% of raw soybean (RS) and with 1% of soaked and boiled soybean (BS). The samples of: duodenum (DUO), proximal jejunum (PROX), mid-jejunum (MID), distal-jejunum (DIST) and ileum (ILE) were collected. The following processes in these samples were evaluated: mitosis (Ki-67), apoptosis (Cpp32), autophagy (MAP I LC3) and DNA damage (p53). Present data show that modification of soybean by soaking and subsequent boiling markedly influences the enterocyte turnover in the small intestine mucosa. Increased mitotic ratio in the intestine of rats fed with boiled soybean masks the negative effects of soybean on the small intestine structure.
Authors:
M M Godlewski; P Slazak; R Zabielski; A Piastowska; M A Gralak
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of physiology and pharmacology : an official journal of the Polish Physiological Society     Volume:  57 Suppl 7     ISSN:  1899-1505     ISO Abbreviation:  J. Physiol. Pharmacol.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2007-01-17     Completed Date:  2007-12-13     Revised Date:  2008-05-19    
Medline Journal Info:
Nlm Unique ID:  9114501     Medline TA:  J Physiol Pharmacol     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  125-33     Citation Subset:  IM    
Affiliation:
Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw Agricultural University, Warsaw, Poland. mickgodl@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Autophagy / physiology*
Cell Growth Processes / physiology
Diet
Dietary Supplements
Enterocytes / cytology*,  metabolism
Intestinal Mucosa / cytology*,  metabolism
Jejunum / cytology*,  metabolism
Rats
Rats, Wistar
Soybeans*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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