Document Detail

Quantitative study of persistence of human norovirus genome in water using TaqMan real-time RT-PCR.
MedLine Citation:
PMID:  17953687     Owner:  NLM     Status:  MEDLINE    
AIMS: To evaluate the persistence of human norovirus (NoV) in different types of water at various temperatures using conventional and TaqMan real-time reverse transcription-PCR (RT-PCR). METHODS AND RESULTS: Water from different sources was spiked with NoV and incubated at different temperatures over a 3-month period. NoV viral RNA was amplified by one-step TaqMan real-time RT-PCR and by conventional two-step RT-PCR. NoV persisted in mineral and tap water for over 2 months at all tested temperatures but disappeared after 100 days. At 4 and -20 degrees C, viral degradation was slower than that at 25 degrees C. In river water and effluent from primary sewage treatment, a slight reduction in viral load was observed after 1 month at 4 degrees C. This is the first demonstration of medium-to-long-term survival of human NoVs in different types of water using TaqMan real-time detection. CONCLUSIONS: NoV genome may persist for long periods of time in different types of water. Quantitative TaqMan real-time RT-PCR is a sensitive system that allows accurate evaluation of the persistence of human NoVs in different water samples. SIGNIFICANCE AND IMPACT OF THE STUDY: Our study is one of the few to demonstrate the ability of NoV to survive for a long time in water.
E S Ngazoa; I Fliss; J Jean
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-10-22
Journal Detail:
Title:  Journal of applied microbiology     Volume:  104     ISSN:  1365-2672     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-02-21     Completed Date:  2008-05-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  707-15     Citation Subset:  IM    
Département des Aliments et de Nutrition, Institut des Nutraceutiques et Aliments Fonctionnels (INAF), Université Laval, Québec, QC, Canada.
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MeSH Terms
Caliciviridae Infections / microbiology*
Gastroenteritis / microbiology*
Genome, Viral
Microbial Viability
Norovirus / physiology*
Reverse Transcriptase Polymerase Chain Reaction / methods
Water Microbiology*
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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