Document Detail


Quantitative study of changes in intestinal morphology and mucus gel on total parenteral nutrition in rats.
MedLine Citation:
PMID:  11104649     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Quantification of changes in gastrointestinal morphology and mucus gel has been difficult to study. In the present study, we investigated changes in rat intestine under total parenteral nutrition (TPN) using fluoresceinated lectin staining and image analysis. Wistar rats (n = 34) were divided into two groups: one group received TPN for 2 weeks, and a control group received standard rat chow and water ad libitum for the same period. A 1-cm segment of distal ileum was removed and cut into cross sections. Sections were stained with hematoxylin and eosin, and to stain the mucus, periodic acid-Schiff (PAS), alcian blue (AB), and fluoresceinated lectin, that is, FITC-labeled Ulex europaeus agglutinin I (FITC-UEA-I), were used. Light microscope images were stored in a personal computer and analyzed using image analysis. We measured perimeter length, mucosal thickness, villus area, villus surface area index, mucus stain-positive area, mucosal area ratio, and mucosal surface area ratio. Perimeter length, mucosal thickness, villus area, and villus surface area index in the TPN group were significantly less than those in the control group (P < 0.001 for each parameter). In all mucus stainings, the stain-positive area in the TPN group was significantly less than that in the control group. However, there were no significant differences in mucosal area or mucosal surface area ratios between the two groups. The FITC-UEA-I-positive area was significantly greater than the PAS- or and AB-positive area. There were significant positive correlations between the FITC-UEA-I-positive area and both the PAS-positive and AB-positive areas. TPN for 2 weeks promoted intestinal atrophy and decreased absolute quantity of mucus gel. We successfully introduced the FITC-UEA-I staining method to evaluate changes in mucus gel.
Authors:
K Sakamoto; H Hirose; A Onizuka; M Hayashi; N Futamura; Y Kawamura; T Ezaki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of surgical research     Volume:  94     ISSN:  0022-4804     ISO Abbreviation:  J. Surg. Res.     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2001-01-18     Completed Date:  2001-01-18     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0376340     Medline TA:  J Surg Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  99-106     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Academic Press.
Affiliation:
First Department of Surgery, Department of Microbiology, Gifu University School of Medicine, Tsukasa-machi 40, Gifu, 500-8076, Japan.
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MeSH Terms
Descriptor/Qualifier:
Alcian Blue
Animals
Coloring Agents
Eosine Yellowish-(YS)
Fluorescein-5-isothiocyanate
Ileum / cytology
Intestinal Mucosa / cytology*
Intestine, Small / cytology*
Lectins
Male
Mucus / cytology*
Nutritional Status
Parenteral Nutrition, Total*
Periodic Acid-Schiff Reaction
Peyer's Patches / cytology,  pathology
Plant Lectins*
Rats
Rats, Wistar
Chemical
Reg. No./Substance:
0/Coloring Agents; 0/Lectins; 0/Plant Lectins; 0/Ulex europaeus lectins; 12040-44-7/Alcian Blue; 17372-87-1/Eosine Yellowish-(YS); 3326-32-7/Fluorescein-5-isothiocyanate

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