Document Detail


Quantitative studies on ferredoxin in greening bean leaves.
MedLine Citation:
PMID:  4360717     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two methods of measuring small amounts of the iron-sulphur protein ferredoxin are described. One involves measurements of the signal at g=1.96 produced by reduced ferredoxin in an e.p.r. (electron-paramagnetic-resonance) spectrometer; the other depends on the rate of ferredoxin-dependent electron transport in a chloroplast bioassay measured in an O(2) electrode. These methods of measurement were used to examine the development of ferredoxin during the greening of etiolated bean leaves. Ferredoxin is present in low concentrations in the leaves and cotyledons of 14-day-old etiolated beans (Phaseolus vulgaris L. var. Canadian Wonder), and develops in a linear manner with time when the leaves are illuminated. This synthesis appears to be independent of chlorophyll synthesis during the early stages of greening. However, the chlorophyll/ferredoxin ratio reaches a final value of approx. 360 irrespective of the light intensity, indicating the existence of a control mechanism operative in deciding the stoicheiometry of these components in the mature chloroplast. The ferredoxin synthesis appears to be light-dependent, and red light is the most effective in its promotion. The effect of red illumination is not reversed by far-red light, indicating the absence of a phytochrome control of ferredoxin synthesis. From experiments using specific inhibitors of chloroplast protein synthesis, it is concluded that ferredoxin is synthesized on cytoplasmic ribosomes.
Authors:
B G Haslett; R Cammack; F R Whatley
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  136     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1973 Nov 
Date Detail:
Created Date:  1974-04-25     Completed Date:  1974-04-25     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  697-703     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Biological Assay
Chlorophyll / metabolism
Chloroplasts
Electrodes
Electron Spin Resonance Spectroscopy
Ferredoxins / analysis*,  biosynthesis
Light
Oxygen
Plants / analysis*,  metabolism
Chemical
Reg. No./Substance:
0/Ferredoxins; 1406-65-1/Chlorophyll; 7782-44-7/Oxygen
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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