Document Detail


Quantitative sandwich ELISA for the determination of lupine (Lupinus spp.) in foods.
MedLine Citation:
PMID:  16028967     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The use of lupine in foods has increased considerably during the past decade, reflected by a corresponding increase in reported lupine-induced allergic incidents. Lupine allergy may arise either by primary sensitization or by clinical cross-reactivity in peanut-allergic persons. Detection of lupine proteins in food has previously been based on the use of patient serum. A novel sandwich enzyme-linked immunosorbent assay (ELISA) for the detection and quantification of lupine in processed foods was developed, using a polyclonal rabbit antilupine capture antibody and a biotinylated conjugate of the same antibody for detection. The antibody was highly specific for lupine, apart from minor cross-reactivities to other legumes. The assay had a detection limit of 1 mug/g and was successfully used to quantify lupine protein in various food matrixes. Recoveries ranged from 60 to 116%, while the intra-and interassay coefficients of variation were <6% and <21%, respectively.
Authors:
Lise Holden; Christiane K Faeste; Eliann Egaas
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  53     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-07-20     Completed Date:  2005-09-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5866-71     Citation Subset:  IM    
Affiliation:
National Veterinary Institute, Post Office Box 8156 Dep., N-0033 Oslo, Norway. lise.holden@vetinst.no
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MeSH Terms
Descriptor/Qualifier:
Antibody Specificity
Enzyme-Linked Immunosorbent Assay / methods*
Food Analysis / methods*
Food Hypersensitivity
Lupinus / chemistry*,  immunology
Plant Proteins / analysis*
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Plant Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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