Document Detail


Quantitative real-time PCR for detection of the neurotoxin gene of Clostridium botulinum type B in equine and bovine samples.
MedLine Citation:
PMID:  22537645     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Clostridium botulinum type B is estimated to cause more than 85% of cases of equine botulism in the United States, as well as many outbreaks in cattle. In this study, a quantitative real-time polymerase chain reaction for detection of the neurotoxin gene of C. botulinum type B was compared to the mouse bioassay using 45 positive and 43 negative samples of equine, bovine or associated environmental origin. The sensitivity of the qPCR assay was 96%, whereas the sensitivity of the mouse bioassay was 84%. The specificity of the qPCR assay was 95% and the specificity of the mouse bioassay was 100%.
Authors:
Amy L Johnson; Raymond W Sweeney; Susan C McAdams; Robert H Whitlock
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-04-24
Journal Detail:
Title:  Veterinary journal (London, England : 1997)     Volume:  194     ISSN:  1532-2971     ISO Abbreviation:  Vet. J.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-11-02     Completed Date:  2013-11-15     Revised Date:  2013-11-25    
Medline Journal Info:
Nlm Unique ID:  9706281     Medline TA:  Vet J     Country:  England    
Other Details:
Languages:  eng     Pagination:  118-20     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Assay / methods
Botulinum Toxins / genetics,  metabolism*
Botulism / diagnosis*,  microbiology
Cattle
Cattle Diseases / diagnosis*,  microbiology
Clostridium botulinum / classification*
Horse Diseases / diagnosis*,  microbiology
Horses
Mice
Real-Time Polymerase Chain Reaction / methods,  veterinary*
Reproducibility of Results
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0Y70779M1F/rimabotulinumtoxinB; EC 3.4.24.69/Botulinum Toxins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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