Document Detail


Quantitative radioautography of sugar transport in intestinal biopsies from normal humans and a patient with glucose-galactose malabsorption.
MedLine Citation:
PMID:  5009124     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Both galactose accumulation and phlorizin binding by columnar epithelial cells have been investigated in vitro with a recently developed technique for high-resolution, plastic-section radioautography which is particularly suited to small quantities of biopsy tissue. Grain density analysis of the radioautographs provides definitive support for the view that the cellular mechanisms underlying glucose-galactose absorption in laboratory animals are fully applicable to the small intestine of man. Even the number of sugar carriers at the microvillar membrane appears similar and the major quantitative difference, lower affinity for phlorizin in man, correlates with the finding that phlorizin is also a less potent inhibitor of uphill, galactose transport at the microvilli. In addition, radioautographs of biopsies taken 2 yr apart from a patient with glucose-galactose malabsorption provide evidence that the cellular defect in this inborn error of transport is a persistent reduction in the number of functioning sugar carriers at the microvillar membrane.
Authors:
C E Stirling; A J Schneider; M D Wong; W B Kinter
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of clinical investigation     Volume:  51     ISSN:  0021-9738     ISO Abbreviation:  J. Clin. Invest.     Publication Date:  1972 Feb 
Date Detail:
Created Date:  1972-04-04     Completed Date:  1972-04-04     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7802877     Medline TA:  J Clin Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  438-51     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Autoradiography
Biopsy
Carbohydrate Metabolism, Inborn Errors / metabolism*,  pathology
Child, Preschool
Galactose / metabolism*
Glucose / metabolism*
Humans
Infant
Intestinal Mucosa / metabolism,  pathology
Intestine, Small / metabolism*,  pathology
Malabsorption Syndromes / metabolism*,  pathology
Membranes / metabolism
Methods
Microscopy, Electron
Phlorhizin / metabolism
Tritium
Chemical
Reg. No./Substance:
10028-17-8/Tritium; 26566-61-0/Galactose; 50-99-7/Glucose; 60-81-1/Phlorhizin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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