| Quantitative proton spectroscopy of canine brain: in vivo and in vitro correlations. | |
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MedLine Citation:
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PMID: 7968436 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Quantitative, single-voxel proton NMR spectroscopy of normal brain was performed in five adult beagle dogs using the cerebral water signal as an internal intensity reference. The same brain regions were then rapidly isolated and frozen using a pneumatic biopsy drill, perchloric acid extracted, and analyzed by biochemical assay and high-resolution NMR spectroscopy. The concentrations of the major resonances in the in vivo and in vitro spectra were compared, and good agreement was found between the different measurements. The in vivo spectra contained three peaks at 3.21, 3.04, and 2.02 ppm, which are usually assigned to trimethylamines (TMA), creatines, and N-acetyl derivatives (NAc), which corresponded to be the following metabolite concentration values: 1.7 +/- 0.6, 7.7 +/- 2.1, and 10.9 +/- 2.7 mumol/g wet weight respectively. In vitro, the following metabolite concentrations were measured: glycerophosphocholine (GPC) 1.3 +/- 0.2, phosphocholine (PC) 0.5 +/- 0.1, phosphocreatine (PCr) 2.6 +/- 0.4, creatine (Cr) 5.9 +/- 1.4, and N-Acetyl aspartate (NAA) 8.9 +/- 1.8 mumol/g wet weight. Therefore, the 3.21 ppm resonance observed in the in vivo spectrum is predominantly GPC and PC in a ratio of 2.6:1, the 3.04 ppm resonance is Cr and PCr in a ratio of 2.3:1, and the 2.02 ppm resonance is predominantly (approximately 80%) NAA with small contributions from N-acetylaspartylglutamate (NAAG) and glutamate. The data presented here validate the technique of water referencing as a simple and convenient means of quantitating single-voxel in vivo proton NMR spectra of the brain. |
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Authors:
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P B Barker; S N Breiter; B J Soher; J C Chatham; J R Forder; M A Samphilipo; C A Magee; J H Anderson |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine Volume: 32 ISSN: 0740-3194 ISO Abbreviation: Magn Reson Med Publication Date: 1994 Aug |
Date Detail:
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Created Date: 1994-12-06 Completed Date: 1994-12-06 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8505245 Medline TA: Magn Reson Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 157-63 Citation Subset: IM |
Affiliation:
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Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, Maryland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aspartic Acid / analogs & derivatives, analysis Brain Chemistry* Dogs Glycerylphosphorylcholine / analysis Magnetic Resonance Spectroscopy* Methylamines / analysis Phosphocreatine / analysis Phosphorylcholine / analysis |
| Grant Support | |
ID/Acronym/Agency:
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R01 CA37870/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Methylamines; 107-73-3/Phosphorylcholine; 56-84-8/Aspartic Acid; 563-24-6/Glycerylphosphorylcholine; 67-07-2/Phosphocreatine; 75-50-3/trimethylamine; 997-55-7/N-acetylaspartate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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