Document Detail

Quantitative proteomic analysis of human osteoblast-like MG-63 cells in response to bioinert implant material titanium and polyetheretherketone.
MedLine Citation:
PMID:  22504627     Owner:  NLM     Status:  Publisher    
Commercially pure titanium (cpTi) and polyetheretherketone (PEEK) are widely used as implant materials in orthopedics and dental therapeutics. However, there is still no comprehensive biocompatibility information of them at molecular level. By employing stable isotope labeling with amino acids in cell culture (SILAC), we profiled the dynamic protein expression changes in human osteoblast-like MG-63 cells cultured on cpTi and PEEK, with TCP as control. About 2000 proteins were quantified and 400 proteins showed apparent alterations in expression levels upon each material treatment. Notably, the protein expression changes became smaller and smaller with prolonged contact, which revealed the adaptive process of osteoblast responding to the bioinert materials. Similar expression changes were observed for both cpTi and PEEK. The representative pathways reflected the regulation of biosynthesis, metabolism and cell adhesion in the adaptive process. In addition, PEEK showed more inhibition effects on mRNA processing, which explained the lower proliferation rate of the cells cultured on PEEK. Our results indicated that the widely used bioinert materials cpTi and PEEK could individually induce a cooperative response including many proteins and even a group of pathways. This study has established a basis for better understanding the biocompatibility of modified implant biomaterials at molecular level.
Minzhi Zhao; Qingsong Wang; Xiaochen Liu; Wenjia Lai; Xuyang Zhao; Mingrui An; Shicheng Wei; Jianguo Ji
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-29
Journal Detail:
Title:  Journal of proteomics     Volume:  -     ISSN:  1876-7737     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-4-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101475056     Medline TA:  J Proteomics     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier B.V.
Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.
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