| Quantitative proteomic analysis of human breast epithelial cells with differential telomere length. | |
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MedLine Citation:
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PMID: 17395154 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Telomeres play important functional roles in cell proliferation, cell cycle regulation, and genetic stability, in which telomere length is critical. In this study, quantitative proteome comparisons for the human breast epithelial cells with short and long telomeres (184-hTERTL vs. 184-hTERTS and 90P-hTERTL vs. 90P-hTERTS), resulting from transfection of the human telomerase reverse transcriptase (hTERT) gene, were performed using cleavable isotope-coded affinity tags. More than 2000 proteins were quantified in each comparative experiment, with approximately 77% of the proteins identified in both analyses. In the cells with long telomeres, significant and consistent alterations were observed in metabolism (amino acid, nucleotide, and lipid metabolism), genetic information transmission (transcription and translation regulation, spliceosome and ribosome complexes), and cell signaling. Interestingly, the DNA excision repair pathway is enhanced, while integrin and its ligands are downregulated in the cells with long telomeres. These results may provide valuable information related to telomere functions. |
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Authors:
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Li-Rong Yu; King C Chan; Hidetoshi Tahara; David A Lucas; Koushik Chatterjee; Haleem J Issaq; Timothy D Veenstra |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2007-03-22 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 356 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2007 May |
Date Detail:
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Created Date: 2007-04-11 Completed Date: 2007-06-28 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 942-7 Citation Subset: IM |
Affiliation:
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Laboratory of Proteomics and Analytical Technologies, SAIC-Frederick, Inc., NCI-Frederick, Frederick, Maryland 21702, USA. lyu@ncifcrf.gov |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Breast
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metabolism*,
ultrastructure Cell Line DNA Repair / physiology* Epithelial Cells / metabolism*, ultrastructure Humans Proteome / metabolism* Telomere / physiology*, ultrastructure |
| Grant Support | |
ID/Acronym/Agency:
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N01 CO12400/CO/NCI/CO/NCI NIH HHS; N01-CO-12400/CO/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Proteome |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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