| Quantitative proteome analysis of the 20S proteasome of apoptotic Jurkat T cells. | |
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MedLine Citation:
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PMID: 20364280 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Regulated proteolysis plays important roles in cell biology and pathological conditions. A crosstalk exists between apoptosis and the ubiquitin-proteasome system, two pathways responsible for regulated proteolysis executed by different proteases. To investigate whether the apoptotic process also affects the 20S proteasome, we performed three independent SILAC-based quantitative proteome approaches: 1-DE/MALDI-MS, small 2-DE/MALDI-MS and large 2-DE/nano-LC-ESI-MS. Taking the results of all experiments together, no quantitative changes were observed for the α- and β-subunits of the 20S proteasome except for subunit α7. This protein was identified in two protein spots with a down-regulation of the more acidic protein species (α7a) and up-regulation of the more basic protein species (α7b) during apoptosis. The difference in these two α7 protein species could be attributed to oxidation of cysteine-41 to cysteine sulfonic acid and phosphorylation at serine-250 near the C terminus in α7a, whereas these modifications were missing in α7b. These results pointed to the biological significance of posttranslational modifications of proteasome subunit α7 after induction of apoptosis. |
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Authors:
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Frank Schmidt; Burkhardt Dahlmann; Hanne K Hustoft; Christian J Koehler; Margarita Strozynski; Alexander Kloss; Ursula Zimny-Arndt; Peter R Jungblut; Bernd Thiede |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-03 |
Journal Detail:
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Title: Amino acids Volume: 41 ISSN: 1438-2199 ISO Abbreviation: Amino Acids Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-05-26 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9200312 Medline TA: Amino Acids Country: Austria |
Other Details:
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Languages: eng Pagination: 351-61 Citation Subset: IM |
Affiliation:
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The Biotechnology Centre of Oslo, University of Oslo, Gaustadalleen 21, Blindern, P.O. Box 1125, 0317, Oslo, Norway. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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