Document Detail


Quantitative profiling of polar glycerolipid species from organs of wild-type Arabidopsis and a phospholipase Dalpha1 knockout mutant.
MedLine Citation:
PMID:  16843506     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lipid profiling is a targeted metabolomics platform that provides a comprehensive analysis of lipid species with high sensitivity. Profiling based on electrospray ionization tandem mass spectrometry (ESI-MS/MS) provides quantitative data and is adaptable to high throughput analyses. Here we report the profiling of 140 apparent molecular species of polar glycerolipids in Arabidopsis leaves, flower stalks, flowers, siliques, roots, and seeds. Considerable differences in lipid species occur among these organs, providing insights into the different lipid metabolic activities in a specific organ. In addition, comparative profiling between wild-type and a knockout mutant pldalpha1 (locus ID: AT3G15730) provides insight into the metabolic function of phospholipase D (PLD) in different organs. PLDalpha1 contributes significantly to phosphatidic acid (PA) levels in roots, seeds, flowers, and flower stalks, but little to basal PA levels in siliques and leaves. In seeds of the pldalpha1 mutant plants, levels of PA, lysophosphatidylcholine, and lysophosphatidylethanolamine were significantly lower than those of wild-type seeds, suggesting a role for PLDalpha1 in membrane lipid degradation in seeds.
Authors:
Shivakumar Pattada Devaiah; Mary R Roth; Ethan Baughman; Maoyin Li; Pamela Tamura; Richard Jeannotte; Ruth Welti; Xuemin Wang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2006-07-14
Journal Detail:
Title:  Phytochemistry     Volume:  67     ISSN:  0031-9422     ISO Abbreviation:  Phytochemistry     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-30     Completed Date:  2007-01-08     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  0151434     Medline TA:  Phytochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1907-24     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Kansas State University, Manhattan, KS 66506, USA.
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / enzymology,  genetics,  metabolism*
Arabidopsis Proteins / genetics,  metabolism
Lipids / analysis*,  chemistry
Molecular Structure
Mutation / genetics*
Phosphatidic Acids / analysis,  chemistry
Phospholipase D / genetics,  metabolism*
Phospholipids / analysis,  chemistry
Plants, Genetically Modified
Principal Component Analysis
Spectrometry, Mass, Electrospray Ionization
Grant Support
ID/Acronym/Agency:
P20 RR016475/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/Lipids; 0/Phosphatidic Acids; 0/Phospholipids; EC 3.1.4.4/Phospholipase D

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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