Document Detail


Quantitative profiling of the pathological prion protein allotypes in bank voles by liquid chromatography-mass spectrometry.
MedLine Citation:
PMID:  17008136     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The conversion of the cellular prion protein (PrP(C)) into a misfolded isoform (PrP(TSE)) that accumulates in the brain of affected individuals is the key feature of transmissible spongiform encephalopaties (TSEs). Susceptibility to TSEs is influenced by polymorphisms of the prion gene suggesting that the presence of certain amino acid residues may facilitate the pathological conversion. In this work, we describe a quantitative, fast and reliable HPLC-MS method that allowed to demonstrate that in the brain of 109(Met/Ile) heterozygous bank voles infected with the mouse adapted scrapie strain 139A, there are comparable amounts of PrP(TSE) with methionine or isoleucine in position 109, suggesting that in this TSE model the two allotypes have similar rates of accumulation. This method can be easily adapted for the quantitative determination of PrP allotypes in the brain of other natural or experimental TSE models.
Authors:
C Cartoni; M E Schininà; B Maras; R Nonno; G Vaccari; M Di Bari; M Conte; A De Pascalis; S Principe; F Cardone; M Pocchiari; U Agrimi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-09-27
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  849     ISSN:  1570-0232     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-04-10     Completed Date:  2007-06-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  302-6     Citation Subset:  IM    
Affiliation:
Istituto Superiore di Sanità, Department of Food Safety and Veterinary Public Health, Viale Regina Elena 299, 00161 Rome, Italy. cartoni@iss.it
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MeSH Terms
Descriptor/Qualifier:
Animals
Arvicolinae
Blotting, Western
Brain / metabolism*,  pathology
Chromatography, High Pressure Liquid / methods*
Mass Spectrometry / methods*
Mice
PrPC Proteins / analysis,  chemistry
PrPSc Proteins / analysis,  chemistry
Prions / analysis,  chemistry*
Chemical
Reg. No./Substance:
0/PrPC Proteins; 0/PrPSc Proteins; 0/Prions

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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