| Quantitative profiling of biomarkers related to B-vitamin status, tryptophan metabolism and inflammation in human plasma by liquid chromatography/tandem mass spectrometry. | |
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MedLine Citation:
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PMID: 19337982 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Vitamins B2 and B6 serve as cofactors in enzymatic reactions involved in tryptophan and homocysteine metabolism. Plasma concentrations of these vitamins and amino acids are related to smoking and inflammation, and correlate with other markers of immune activation. Large-scale studies of these relations have been hampered by lack of suitable analytical methods. The assay described includes riboflavin, five vitamin B6 forms (pyridoxal 5'-phosphate, pyridoxal, 4-pyridoxic acid, pyridoxine and pyridoxamine), tryptophan and six tryptophan metabolites (kynurenine, kynurenic acid, anthranilic acid, 3-hydroxykynurenine, xanthurenic acid and 3-hydroxyanthranilic acid), cystathionine, neopterin and cotinine. Trichloroacetic acid containing 13 isotope-labelled internal standards was added to 60 microL of plasma, the mixture was centrifuged, and the resulting supernatant used for analysis. The analytes were separated within 5 min on a stable-bond C8 column by a gradient-type mobile phase containing acetonitrile, heptafluorobutyric acid and high concentration (650 mmol/L) of acetic acid, and detected using electrospray ionization tandem mass spectrometry (ESI-MS/MS). The mobile phase ensured sufficient separation and high ionization efficiency of all analytes. Recoveries were 75-123% and within-day and between-day coefficients of variance (CVs) were 2.5-9.5% and 5.4-16.9%, respectively. Limits of detection ranged from 0.05 to 7 nmol/L. The method enables quantification of endogenous plasma concentrations of 16 analytes related to B-vitamin status and inflammation, and may prove useful in large-scale epidemiological studies. |
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Authors:
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Øivind Midttun; Steinar Hustad; Per M Ueland |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Validation Studies |
Journal Detail:
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Title: Rapid communications in mass spectrometry : RCM Volume: 23 ISSN: 0951-4198 ISO Abbreviation: Rapid Commun. Mass Spectrom. Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-04-13 Completed Date: 2009-06-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8802365 Medline TA: Rapid Commun Mass Spectrom Country: England |
Other Details:
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Languages: eng Pagination: 1371-9 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2009 John Wiley & Sons, Ltd. |
Affiliation:
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Bevital A/S, Armauer Hansens Hus, N-5021 Bergen, Norway. nkjbm@uib.no |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biological Markers
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blood* Chromatography, Liquid / methods* Cotinine / blood Cystathionine / blood Humans Inflammation / blood* Neopterin / blood Riboflavin / blood Sensitivity and Specificity Tandem Mass Spectrometry / methods* Tryptophan / blood*, metabolism Vitamin B 6 / blood* |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 486-56-6/Cotinine; 56-88-2/Cystathionine; 670-65-5/Neopterin; 73-22-3/Tryptophan; 8059-24-3/Vitamin B 6; 83-88-5/Riboflavin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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