Document Detail

Quantitative profiling of bile acids in biofluids and tissues based on accurate mass high resolution LC-FT-MS: compound class targeting in a metabolomics workflow.
MedLine Citation:
PMID:  18511357     Owner:  NLM     Status:  MEDLINE    
We report a sensitive, generic method for quantitative profiling of bile acids and other endogenous metabolites in small quantities of various biological fluids and tissues. The method is based on a straightforward sample preparation, separation by reversed-phase high performance liquid-chromatography mass spectrometry (HPLC-MS) and electrospray ionisation in the negative ionisation mode (ESI-). Detection is performed in full scan using the linear ion trap Fourier transform mass spectrometer (LTQ-FTMS) generating data for many (endogenous) metabolites, not only bile acids. A validation of the method in urine, plasma and liver was performed for 17 bile acids including their taurine, sulfate and glycine conjugates. The method is linear in the 0.01-1 microM range. The accuracy in human plasma ranges from 74 to 113%, in human urine 77 to 104% and in mouse liver 79 to 140%. The precision ranges from 2 to 20% for pooled samples even in studies with large number of samples (n>250). The method was successfully applied to a multi-compartmental APOE*3-Leiden mouse study, the main goal of which was to analyze the effect of increasing dietary cholesterol concentrations on hepatic cholesterol homeostasis and bile acid synthesis. Serum and liver samples from different treatment groups were profiled with the new method. Statistically significant differences between the diet groups were observed regarding total as well as individual bile acid concentrations.
Ivana Bobeldijk; Maarten Hekman; Jitske de Vries-van der Weij; Leon Coulier; Raymond Ramaker; Robert Kleemann; Teake Kooistra; Carina Rubingh; Andreas Freidig; Elwin Verheij
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Validation Studies     Date:  2008-05-15
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  871     ISSN:  1570-0232     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-08-12     Completed Date:  2008-10-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  306-13     Citation Subset:  IM    
TNO Quality of Life, Analytical Research, Utrechtseweg 48, 3700 AJ Zeist, The Netherlands.
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MeSH Terms
Bile Acids and Salts / analysis*,  blood,  urine
Cholesterol, Dietary / administration & dosage,  pharmacology
Chromatography, Liquid / methods*
Computational Biology / methods*
Fourier Analysis
Liver / chemistry,  drug effects
Mass Spectrometry / methods*
Reproducibility of Results
Reg. No./Substance:
0/Bile Acids and Salts; 0/Cholesterol, Dietary

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