Document Detail


Quantitative polarized Raman spectroscopy in highly turbid bone tissue.
MedLine Citation:
PMID:  20615030     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Polarized Raman spectroscopy allows measurement of molecular orientation and composition and is widely used in the study of polymer systems. Here, we extend the technique to the extraction of quantitative orientation information from bone tissue, which is optically thick and highly turbid. We discuss multiple scattering effects in tissue and show that repeated measurements using a series of objectives of differing numerical apertures can be employed to assess the contributions of sample turbidity and depth of field on polarized Raman measurements. A high numerical aperture objective minimizes the systematic errors introduced by multiple scattering. We test and validate the use of polarized Raman spectroscopy using wild-type and genetically modified (oim/oim model of osteogenesis imperfecta) murine bones. Mineral orientation distribution functions show that mineral crystallites are not as well aligned (p<0.05) in oim/oim bones (28+/-3 deg) compared to wild-type bones (22+/-3 deg), in agreement with small-angle X-ray scattering results. In wild-type mice, backbone carbonyl orientation is 76+/-2 deg and in oim/oim mice, it is 72+/-4 deg (p>0.05). We provide evidence that simultaneous quantitative measurements of mineral and collagen orientations on intact bone specimens are possible using polarized Raman spectroscopy.
Authors:
Mekhala Raghavan; Nadder D Sahar; Robert H Wilson; Mary-Ann Mycek; Nancy Pleshko; David H Kohn; Michael D Morris
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  15     ISSN:  1560-2281     ISO Abbreviation:  J Biomed Opt     Publication Date:    2010 May-Jun
Date Detail:
Created Date:  2010-07-09     Completed Date:  2010-10-29     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  037001     Citation Subset:  IM    
Affiliation:
University of Michigan, Department of Biomedical Engineering, 930 North University Avenue, Room 4638, Ann Arbor, Michigan 48109, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bone and Bones / chemistry*
Collagen Type I / chemistry
Disease Models, Animal
Elasticity
Female
Light
Mice
Minerals / chemistry
Nephelometry and Turbidimetry
Osteogenesis Imperfecta / genetics
Reproducibility of Results
Scattering, Radiation
Spectrum Analysis, Raman / methods*
Tibia / chemistry*
Grant Support
ID/Acronym/Agency:
R01 AR047969/AR/NIAMS NIH HHS; R01 AR048337/AR/NIAMS NIH HHS; R01 AR055222/AR/NIAMS NIH HHS; R01-AR052010/AR/NIAMS NIH HHS; R01-AR48337/AR/NIAMS NIH HHS; R01-CA-114542/CA/NCI NIH HHS; R90-DK071506/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Collagen Type I; 0/Minerals
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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