| Quantitative polarized Raman spectroscopy in highly turbid bone tissue. | |
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MedLine Citation:
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PMID: 20615030 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Polarized Raman spectroscopy allows measurement of molecular orientation and composition and is widely used in the study of polymer systems. Here, we extend the technique to the extraction of quantitative orientation information from bone tissue, which is optically thick and highly turbid. We discuss multiple scattering effects in tissue and show that repeated measurements using a series of objectives of differing numerical apertures can be employed to assess the contributions of sample turbidity and depth of field on polarized Raman measurements. A high numerical aperture objective minimizes the systematic errors introduced by multiple scattering. We test and validate the use of polarized Raman spectroscopy using wild-type and genetically modified (oim/oim model of osteogenesis imperfecta) murine bones. Mineral orientation distribution functions show that mineral crystallites are not as well aligned (p<0.05) in oim/oim bones (28+/-3 deg) compared to wild-type bones (22+/-3 deg), in agreement with small-angle X-ray scattering results. In wild-type mice, backbone carbonyl orientation is 76+/-2 deg and in oim/oim mice, it is 72+/-4 deg (p>0.05). We provide evidence that simultaneous quantitative measurements of mineral and collagen orientations on intact bone specimens are possible using polarized Raman spectroscopy. |
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Authors:
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Mekhala Raghavan; Nadder D Sahar; Robert H Wilson; Mary-Ann Mycek; Nancy Pleshko; David H Kohn; Michael D Morris |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Journal of biomedical optics Volume: 15 ISSN: 1560-2281 ISO Abbreviation: J Biomed Opt Publication Date: 2010 May-Jun |
Date Detail:
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Created Date: 2010-07-09 Completed Date: 2010-10-29 Revised Date: 2013-05-29 |
Medline Journal Info:
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Nlm Unique ID: 9605853 Medline TA: J Biomed Opt Country: United States |
Other Details:
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Languages: eng Pagination: 037001 Citation Subset: IM |
Affiliation:
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University of Michigan, Department of Biomedical Engineering, 930 North University Avenue, Room 4638, Ann Arbor, Michigan 48109, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bone and Bones / chemistry* Collagen Type I / chemistry Disease Models, Animal Elasticity Female Light Mice Minerals / chemistry Nephelometry and Turbidimetry Osteogenesis Imperfecta / genetics Reproducibility of Results Scattering, Radiation Spectrum Analysis, Raman / methods* Tibia / chemistry* |
| Grant Support | |
ID/Acronym/Agency:
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R01 AR047969/AR/NIAMS NIH HHS; R01 AR048337/AR/NIAMS NIH HHS; R01 AR055222/AR/NIAMS NIH HHS; R01-AR052010/AR/NIAMS NIH HHS; R01-AR48337/AR/NIAMS NIH HHS; R01-CA-114542/CA/NCI NIH HHS; R90-DK071506/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Collagen Type I; 0/Minerals |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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