| Quantitative morphometry of respiratory tract epithelial cells as a tool for testing chemopreventive agent efficacy. | |
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MedLine Citation:
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PMID: 20392991 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previously, we developed a 30-day transformation assay (focus inhibition, FIA) of rat tracheal epithelial (RTE) cells to identify cancer preventive agents. This study reports nuclear density (ND) as a morphometric biomarker for efficacy evaluation of at an early stage before transformed foci appear. Positive (oltipraz, D-carvone, fumaric acid, and 2-amino-4-methylpyridine or 2-A-MPR) and negative agents (myristoleic acid, anethole trithione, hydrocortisone, and 3'-hydroxyflavanone), identified from FIA, were tested for their effect on ND. RTE cells plated for 24 h were treated with a carcinogen, benzo[a] pyrene (B[a]P) or plus a test agent. The data based on the number of nuclei in agent-treated and control cells at day 14 showed that all FIA-positive agents inhibited ND from 23-66% at 0.3-1000.0 microM and except for myristoleic acid, all of the FIA-negative compounds were also negative in the morphometry assay. As there is strong correlation between the FIA and morphometry data, morphometry analysis is useful for rapid screening of potential chemopreventive agents. |
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Authors:
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Sheela Sharma; Pu Gao; Vernon E Steele |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Anticancer research Volume: 30 ISSN: 1791-7530 ISO Abbreviation: Anticancer Res. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-04-15 Completed Date: 2010-05-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8102988 Medline TA: Anticancer Res Country: Greece |
Other Details:
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Languages: eng Pagination: 737-42 Citation Subset: IM |
Affiliation:
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Division of Toxicology and Preclinical Studies, The Hamner Institutes for Health Sciences, P.O. Box 12137, Research Triangle Park, NC 27709, USA. ssharma@thehamner.org. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anticarcinogenic Agents / pharmacology* Cell Nucleus / drug effects, pathology Cell Transformation, Neoplastic / drug effects*, pathology Drug Screening Assays, Antitumor / methods Epithelial Cells / cytology, drug effects, pathology Male Rats Rats, Inbred F344 Trachea / cytology*, drug effects*, pathology Tracheal Neoplasms / pathology, prevention & control* |
| Grant Support | |
ID/Acronym/Agency:
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N01-CN-43301/CN/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Anticarcinogenic Agents |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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