Document Detail

Quantitative morphometry of respiratory tract epithelial cells as a tool for testing chemopreventive agent efficacy.
MedLine Citation:
PMID:  20392991     Owner:  NLM     Status:  MEDLINE    
Previously, we developed a 30-day transformation assay (focus inhibition, FIA) of rat tracheal epithelial (RTE) cells to identify cancer preventive agents. This study reports nuclear density (ND) as a morphometric biomarker for efficacy evaluation of at an early stage before transformed foci appear. Positive (oltipraz, D-carvone, fumaric acid, and 2-amino-4-methylpyridine or 2-A-MPR) and negative agents (myristoleic acid, anethole trithione, hydrocortisone, and 3'-hydroxyflavanone), identified from FIA, were tested for their effect on ND. RTE cells plated for 24 h were treated with a carcinogen, benzo[a] pyrene (B[a]P) or plus a test agent. The data based on the number of nuclei in agent-treated and control cells at day 14 showed that all FIA-positive agents inhibited ND from 23-66% at 0.3-1000.0 microM and except for myristoleic acid, all of the FIA-negative compounds were also negative in the morphometry assay. As there is strong correlation between the FIA and morphometry data, morphometry analysis is useful for rapid screening of potential chemopreventive agents.
Sheela Sharma; Pu Gao; Vernon E Steele
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Anticancer research     Volume:  30     ISSN:  1791-7530     ISO Abbreviation:  Anticancer Res.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-04-15     Completed Date:  2010-05-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  737-42     Citation Subset:  IM    
Division of Toxicology and Preclinical Studies, The Hamner Institutes for Health Sciences, P.O. Box 12137, Research Triangle Park, NC 27709, USA.
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MeSH Terms
Anticarcinogenic Agents / pharmacology*
Cell Nucleus / drug effects,  pathology
Cell Transformation, Neoplastic / drug effects*,  pathology
Drug Screening Assays, Antitumor / methods
Epithelial Cells / cytology,  drug effects,  pathology
Rats, Inbred F344
Trachea / cytology*,  drug effects*,  pathology
Tracheal Neoplasms / pathology,  prevention & control*
Grant Support
Reg. No./Substance:
0/Anticarcinogenic Agents

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