| Quantitative microanalysis of cell walls of Saprolegnia diclina Humphrey and Tremella mesenterica Fries. | |
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MedLine Citation:
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PMID: 953025 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell walls of the fungi Saprolegnia declina Humphrey and Tremella mesenterica Fries were analyzed quantitatively. Particular attention was paid to the hydrolysis and analysis of neutral sugars, amino sugars and amino acids. These components, together with total lipids, total uronic acids and the ashed residue, accounted for more than 90% by weight of the original dry cell wall preparation. There were substantial losses of amino acids during hydrolysis; however, analytical recovery approached 100% when total protein was calculated from the total nitrogen analysis. The analytical procedures were reproducible (+/- 3% for amino acids and amino sugars, and +/- 5-10% for other components) when applied to individual cell wall preparations. However, even under carefully standardized conditions, different cell wall preparations from the same species showed variable composition. Glucose was the predominant neutral sugar in the cell wall polymers of both species. The amino acid compositions were remarkable in that neither species contained detectable levels of cyst(e)ine. Hydroxyproline was detected in both species. The report from Tremella mesenterica is the first for this amino acid from the cell wall of a Basidiomycete. |
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Authors:
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D S Cameron; I E Taylor |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 444 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1976 Aug |
Date Detail:
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Created Date: 1976-11-01 Completed Date: 1976-11-01 Revised Date: 2000-12-18 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 212-22 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis Basidiomycota / analysis* Cell Wall / analysis* Fungi / analysis* Glucosamine / analysis Glucose / analysis Hexoses / analysis Hydroxyproline / analysis Lipids / analysis Oomycetes / analysis* Polysaccharides / analysis Uronic Acids / analysis |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Hexoses; 0/Lipids; 0/Polysaccharides; 0/Uronic Acids; 3416-24-8/Glucosamine; 50-99-7/Glucose; 51-35-4/Hydroxyproline |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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