Document Detail

Quantitative measurements of released amines from individual exocytosis events.
MedLine Citation:
PMID:  9396001     Owner:  NLM     Status:  MEDLINE    
Chemical analysis of single cells is an area of great interest in the biological sciences. Single-cell systems are being utilized as a model to understand in vivo processes better. One method that is moving to the forefront in cellular analysis is electrochemistry. Owing to their rapid response time and small dimensions, voltammetric microelectrode techniques, such as amperometry and fast-scan voltammetry, have made it possible to monitor minute amounts of biological compounds and transiently occurring chemical events in cellular systems. The application of these methods to the quantitation of individual vesicular release events from single cells is overviewed here. The application of electrochemical monitoring to several types of cultured cells, including bovine adrenal chromaffin cells, rat pheochromocytoma (PC12) cells, beige mouse mast cells, superior cervical ganglion neurons, and human pancreatic beta-cells, as well as to the invertebrate systems, the leech Hirudo medicinalis, and pond snail Planorbis corneus has provided a wealth of new information concerning exocytosis. Results obtained from the studies highlight the potential of electrochemical techniques in cellular analysis to contribute to our understanding of molecular and pharmacological effects on exocytosis. This article overviews work done on all the above cell types with an emphasis on PC12 cells.
R A Clark; A G Ewing
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Review    
Journal Detail:
Title:  Molecular neurobiology     Volume:  15     ISSN:  0893-7648     ISO Abbreviation:  Mol. Neurobiol.     Publication Date:  1997 Aug 
Date Detail:
Created Date:  1998-01-15     Completed Date:  1998-01-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8900963     Medline TA:  Mol Neurobiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1-16     Citation Subset:  IM    
Department of Chemistry, Pennsylvania State University, University Park 16802, USA.
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MeSH Terms
Adrenal Gland Neoplasms
Adrenal Medulla / physiology
Catecholamines / analysis,  secretion*
Cells, Cultured
Chromaffin Cells / physiology
Electrochemistry / methods
Islets of Langerhans / physiology
Mast Cells / physiology
Mice, Mutant Strains
Neurons / physiology
PC12 Cells
Superior Cervical Ganglion / physiology
Reg. No./Substance:

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