Document Detail


Quantitative measurements of integrin-mediated adhesion to extracellular matrix.
MedLine Citation:
PMID:  17697877     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Integrin-mediated adhesion is based on the binding of integrins to immobilized ligands either in the extracellular matrix or on the surface of adjacent cells. The strength of adhesion is determined primarily by the number of adhesive bonds that form. Integrins have also been described as signaling receptors. Like adhesion, signals from integrins receptors can depend on the number of integrins that are bound to substrate attached ligands. The common methods for measuring cell adhesion are only capable of measuring initial interactions because the multivalent nature of adhesive bonds when the cell is considered as a unit, these assays reach plateau levels. To measure adhesive integrin-ligand bonds, a spinning disc device is described in which the mean cell detachment force is proportional to the number of adhesive bonds. Particularly as the field has moved from identification of integrins and their ligands into the analysis of adhesion and its relationship to cell signaling, it is important to shift to assays that relate the extracellular binding to the intracellular signals. Specific plans, methods, and analytic strategies are provided to apply this technology to current problems in integrin biology. At present, this is the only published approach that will provide good measures of the number of adhesive bonds that can be generally applied.
Authors:
David Boettiger
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Review    
Journal Detail:
Title:  Methods in enzymology     Volume:  426     ISSN:  0076-6879     ISO Abbreviation:  Meth. Enzymol.     Publication Date:  2007  
Date Detail:
Created Date:  2007-08-16     Completed Date:  2007-10-11     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  0212271     Medline TA:  Methods Enzymol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1-25     Citation Subset:  IM    
Affiliation:
Department of Microbiology, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Adhesion
Extracellular Matrix / metabolism*
Humans
Integrins / metabolism*
Grant Support
ID/Acronym/Agency:
GM 49866/GM/NIGMS NIH HHS; GM 57388/GM/NIGMS NIH HHS; R01 CA 16502/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Integrins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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