Document Detail


Quantitative measurements of amino acids by terahertz time-domain transmission spectroscopy.
MedLine Citation:
PMID:  16878878     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The quantitative analysis of amino acids by terahertz (THz) time-domain absorption spectroscopy is demonstrated. The optical densities of the amino acids were found to be linearly proportional to the concentration. The molar absorption coefficients of L-glutamic acid (L-Glu), L-glutamic acid sodium salt (Na-L-Glu), L-glutamic acid hydrochloric salt (HCl-L-Glu), L-cysteine (L-Cys), and L-histidine (L-His) were calculated by averaging the THz spectra of the amino acids at several different concentrations in approximately the 0.2-1.0 mol L(-1) range. The concentrations of L-Glu, L-Cys, and L-His mixed samples were successfully calculated with errors of less than 11% and 20% when their concentrations were higher than 0.45 and 0.22 mol L(-1), respectively, by using the obtained molar absorption coefficient.
Authors:
Yuko Ueno; Rakchanok Rungsawang; Isao Tomita; Katsuhiro Ajito
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical chemistry     Volume:  78     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-08-01     Completed Date:  2007-06-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5424-8     Citation Subset:  IM    
Affiliation:
NTT Basic Research Laboratories, 3-1 Morinosato Wakamiya, Atsugi, Kanagawa 243-0198 Japan. ueno@aecl.ntt.co.jp
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis*
Lasers
Sensitivity and Specificity
Spectrum Analysis / instrumentation,  methods*
Time Factors
Chemical
Reg. No./Substance:
0/Amino Acids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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