| Quantitative measurement of carbohydrate binding activity of mouse macrophage lectin. | |
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MedLine Citation:
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PMID: 8176236 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A simple ELISA assay measuring lectin activity of a mouse macrophage galactose/N-acetylgalactosamine-specific C-type lectin (MMGL) was developed. The binding of galactosylated poly-lysine (termed a ligand) to the immobilized soluble form of MMGL (rML) was measured quantitatively. Consistent with the characteristics of MMGL, the binding was calcium dependent and inhibited by galactose and N-acetylgalactosamine. An antiserum against rML inhibited the ligand binding, demonstrating the usefulness of this method for the screening of blocking antibodies. Using this assay, we found a significant interaction between MMGL and carrageenans, a group of sulfated polygalactans. The inhibitory effect of carrageenans was not attributable to a nonspecific interaction because other types of sulfated polysaccharides, such as glycosaminoglycans and fucoidin, did not interfere with the ligand binding. The relevance of the present finding to the biological activities of carrageenans is discussed. |
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Authors:
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Y Imai; T Irimura |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of immunological methods Volume: 171 ISSN: 0022-1759 ISO Abbreviation: J. Immunol. Methods Publication Date: 1994 May |
Date Detail:
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Created Date: 1994-06-08 Completed Date: 1994-06-08 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 1305440 Medline TA: J Immunol Methods Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 23-31 Citation Subset: IM |
Affiliation:
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Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylgalactosamine
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metabolism,
pharmacology Animals Asialoglycoproteins / metabolism Binding, Competitive Calcium Carbohydrate Metabolism* Carrageenan / pharmacology Carrier Proteins / metabolism* Enzyme-Linked Immunosorbent Assay Female Galactose / metabolism, pharmacology Hydrogen-Ion Concentration Kinetics Lectins / metabolism* Lectins, C-Type* Macrophages / metabolism* Membrane Proteins* Mice Polylysine / metabolism Rabbits Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Asialoglycoproteins; 0/Carrier Proteins; 0/Lectins; 0/Lectins, C-Type; 0/Membrane Proteins; 0/Mgl1 protein, mouse; 25104-18-1/Polylysine; 26566-61-0/Galactose; 31022-50-1/Acetylgalactosamine; 7440-70-2/Calcium; 9000-07-1/Carrageenan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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