Document Detail


Quantitative measurement of carbohydrate binding activity of mouse macrophage lectin.
MedLine Citation:
PMID:  8176236     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A simple ELISA assay measuring lectin activity of a mouse macrophage galactose/N-acetylgalactosamine-specific C-type lectin (MMGL) was developed. The binding of galactosylated poly-lysine (termed a ligand) to the immobilized soluble form of MMGL (rML) was measured quantitatively. Consistent with the characteristics of MMGL, the binding was calcium dependent and inhibited by galactose and N-acetylgalactosamine. An antiserum against rML inhibited the ligand binding, demonstrating the usefulness of this method for the screening of blocking antibodies. Using this assay, we found a significant interaction between MMGL and carrageenans, a group of sulfated polygalactans. The inhibitory effect of carrageenans was not attributable to a nonspecific interaction because other types of sulfated polysaccharides, such as glycosaminoglycans and fucoidin, did not interfere with the ligand binding. The relevance of the present finding to the biological activities of carrageenans is discussed.
Authors:
Y Imai; T Irimura
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of immunological methods     Volume:  171     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  1994 May 
Date Detail:
Created Date:  1994-06-08     Completed Date:  1994-06-08     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  23-31     Citation Subset:  IM    
Affiliation:
Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Acetylgalactosamine / metabolism,  pharmacology
Animals
Asialoglycoproteins / metabolism
Binding, Competitive
Calcium
Carbohydrate Metabolism*
Carrageenan / pharmacology
Carrier Proteins / metabolism*
Enzyme-Linked Immunosorbent Assay
Female
Galactose / metabolism,  pharmacology
Hydrogen-Ion Concentration
Kinetics
Lectins / metabolism*
Lectins, C-Type*
Macrophages / metabolism*
Membrane Proteins*
Mice
Polylysine / metabolism
Rabbits
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Asialoglycoproteins; 0/Carrier Proteins; 0/Lectins; 0/Lectins, C-Type; 0/Membrane Proteins; 0/Mgl1 protein, mouse; 25104-18-1/Polylysine; 26566-61-0/Galactose; 31022-50-1/Acetylgalactosamine; 7440-70-2/Calcium; 9000-07-1/Carrageenan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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