Document Detail


Quantitative karyotyping of human chromosomes by dual beam flow cytometry.
MedLine Citation:
PMID:  6961457     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dual beam flow cytometry of chromosomes stained with Hoechst 33258 and chromomycin A3 has been proposed as a method for quantitative classification of human chromosomes (bivariate flow karyotyping). In this paper we investigate the sources and magnitudes of variability in the mean fluorescence intensities of each chromosome group resolved in bivariate flow karyotypes and study the impact of this variability on chromosome classification. Replicate bivariate flow karyotypes of chromosomes isolated from lymphocytes from 10 individuals demonstrated that person-to-person variability was significantly greater than run-to-run variability. The total variability was sufficiently small that it did not interfere with classification of normal chromosome types except chromosomes 9 through 12 and chromosomes 14 and 15. Furthermore, the variability was generally smaller than 1/600th of the mitotic genome, so that one-band rearrangements should be detectable in bivariate flow karyotypes.
Authors:
R G Langlois; L C Yu; J W Gray; A V Carrano
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  79     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1982 Dec 
Date Detail:
Created Date:  1983-03-24     Completed Date:  1983-03-24     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7876-80     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Flow Cytometry / methods*
Fluorescent Dyes
Humans
Karyotyping*
Polymorphism, Genetic
Chemical
Reg. No./Substance:
0/Fluorescent Dyes
Comments/Corrections

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