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Quantitative intact proteomics investigations of alveolar macrophages in sarcoidosis.
MedLine Citation:
PMID:  23060632     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Alveolar macrophages (AMs) are important for granuloma formation, which is the histological hallmark in sarcoidosis. Their function as antigen presenting cell in sarcoidosis is also believed to be relevant to the outcome of disease, resulting either in remission or a prolonged chronic inflammation in the lungs.To study alterations in the expression levels of the soluble proteome of AMs in pulmonary sarcoidosis as compared to healthy controls, with the goal of identifying specific proteins and pathways important for the mechanisms of disease and/or disease phenotype.Quantitative proteomics approach using two-dimensional Difference Gel Electrophoresis coupled to mass spectrometry was applied. Data was evaluated using multivariate modelling and pathway analyses.Results: Sixty-nine protein spots were found to be significant altered between sarcoidosis and healthy controls. Among these, twenty-five unique proteins were identified. Several of the identified proteins were related to key AM functionality, including the Fcγ-mediated phagocytosis and Clathrin-mediated endocytosis pathways.Global proteomics analysis provided identification of alterations of a subset of proteins not previously reported in sarcoidosis. These alterations primarily affect biological pathways related to phagocytic macrophage functionality. These findings provide important insights into the role of macrophages in sarcoidosis pathogenesis.
Authors:
Ernesto Silva; Serhiy Souchelnytskyi; Kie Kasuga; Anders Eklund; Johan Grunewald; Asa M Wheelock
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-11
Journal Detail:
Title:  The European respiratory journal : official journal of the European Society for Clinical Respiratory Physiology     Volume:  -     ISSN:  1399-3003     ISO Abbreviation:  Eur. Respir. J.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8803460     Medline TA:  Eur Respir J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Division of Respiratory Medicine, Karolinska Institutet, Stockholm, Sweden.
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