Document Detail


Quantitative in vivo magnetic resonance spectroscopy using synthetic signal injection.
MedLine Citation:
PMID:  21203385     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Accurate conversion of magnetic resonance spectra to quantitative units of concentration generally requires compensation for differences in coil loading conditions, the gains of the various receiver amplifiers, and rescaling that occurs during post-processing manipulations. This can be efficiently achieved by injecting a precalibrated, artificial reference signal, or pseudo-signal into the data. We have previously demonstrated, using in vitro measurements, that robust pseudo-signal injection can be accomplished using a second coil, called the injector coil, properly designed and oriented so that it couples inductively with the receive coil used to acquire the data. In this work, we acquired nonlocalized phosphorous magnetic resonance spectroscopy measurements from resting human tibialis anterior muscles and used pseudo-signal injection to calculate the Pi, PCr, and ATP concentrations. We compared these results to parallel estimates of concentrations obtained using the more established phantom replacement method. Our results demonstrate that pseudo-signal injection using inductive coupling provides a robust calibration factor that is immune to coil loading conditions and suitable for use in human measurements. Having benefits in terms of ease of use and quantitative accuracy, this method is feasible for clinical use. The protocol we describe could be readily translated for use in patients with mitochondrial disease, where sensitive assessment of metabolite content could improve diagnosis and treatment.
Authors:
Kenneth I Marro; Donghoon Lee; Eric G Shankland; C Mark Mathis; Cecil E Hayes; Seth D Friedman; Martin J Kushmerick
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-12-28
Journal Detail:
Title:  PloS one     Volume:  5     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2010  
Date Detail:
Created Date:  2011-01-04     Completed Date:  2011-07-05     Revised Date:  2011-07-20    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e15166     Citation Subset:  IM    
Affiliation:
Department of Radiology, University of Washington, Seattle, Washington, United States of America. marro@u.washington.edu
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / chemistry
Calibration
Humans
Image Processing, Computer-Assisted / methods*
Magnetic Resonance Spectroscopy / methods*
Models, Biological
Models, Statistical
Muscle, Skeletal / metabolism
Phantoms, Imaging
Phosphates / chemistry
Phosphorus / chemistry
Reproducibility of Results
Grant Support
ID/Acronym/Agency:
R21EB008166/EB/NIBIB NIH HHS; R21EB009438/EB/NIBIB NIH HHS
Chemical
Reg. No./Substance:
0/Phosphates; 56-65-5/Adenosine Triphosphate; 7723-14-0/Phosphorus
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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