Document Detail


Quantitative in vivo imaging of entire embryos with Digital Scanned Laser Light Sheet Fluorescence Microscopy.
MedLine Citation:
PMID:  19375303     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The observation of biological processes in their natural in vivo context is a key requirement for quantitative experimental studies in the life sciences. In many instances, it will be crucial to achieve high temporal and spatial resolution over long periods of time without compromising the physiological development of the specimen. Here, we discuss the principles underlying light sheet-based fluorescence microscopes. The most recent implementation DSLM is a tool optimized to deliver quantitative data for entire embryos at high spatio-temporal resolution. We compare DSLM to the two established light microscopy techniques: confocal and two-photon fluorescence microscopy. DSLM provides up to 50 times higher imaging speeds and a 10-100 times higher signal-to-noise ratio, while exposing the specimens to at least three orders of magnitude less light energy than confocal and two-photon fluorescence microscopes. We conclude with a perspective for future development.
Authors:
Philipp J Keller; Ernst H K Stelzer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2009-04-15
Journal Detail:
Title:  Current opinion in neurobiology     Volume:  18     ISSN:  1873-6882     ISO Abbreviation:  Curr. Opin. Neurobiol.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2009-05-15     Completed Date:  2009-06-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9111376     Medline TA:  Curr Opin Neurobiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  624-32     Citation Subset:  IM    
Affiliation:
Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany. keller@embl.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Fetus / anatomy & histology*
Humans
Image Processing, Computer-Assisted
Lasers
Microscopy, Confocal
Microscopy, Fluorescence / methods*
Microscopy, Fluorescence, Multiphoton
Photobleaching

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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