Document Detail


Quantitative imaging of the tissue contrast agent [Gd(DTPA)](2-) in articular cartilage by laser ablation inductively coupled plasma mass spectrometry.
MedLine Citation:
PMID:  23281293     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is an emerging analytical technique in the generation of quantitative images of MR contrast agent distribution in thin tissue sections of articular cartilage. An analytical protocol is described that includes sample preparation by cryo-cutting of tissue sections, mass spectrometric measurements by LA-ICP-MS and quantification of gadolinium images by one-point calibration, standard addition method (employing matrix-matched laboratory standards) and isotope dilution analysis using highly enriched stable Gd-155 isotope (abundance 92 vs 14.8% in the [Gd(DTPA)](2-) contrast agent). The tissue contrast agent concentrations of [Gd(DTPA)](2-) in cartilage measured in this work are in agreement with findings obtained by magnetic resonance imaging and other analytical methodologies. The LA-ICP-MS imaging data also confirm the observation that the spatial distribution of [Gd(DTPA)](2-) in the near-equilibrium state is highly inhomogeneous across cartilage thickness with the highest concentration measured in superficial cartilage and a strong decrease toward the subchondral bone. In the present work, it is shown for the first time that LA-ICP-MS can be applied to validate the results from quantitative gadolinium-enhanced MRI technique of articular cartilage. Copyright © 2012 John Wiley & Sons, Ltd.
Authors:
Alessandra Sussulini; Edzard Wiener; Tim Marnitz; Bei Wu; Berit Müller; Bernd Hamm; J Sabine Becker
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Contrast media & molecular imaging     Volume:  8     ISSN:  1555-4317     ISO Abbreviation:  Contrast Media Mol Imaging     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-01-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101286760     Medline TA:  Contrast Media Mol Imaging     Country:  United States    
Other Details:
Languages:  eng     Pagination:  204-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 John Wiley & Sons, Ltd.
Affiliation:
Central Division of Analytical Chemistry, Forschungszentrum Jülich, D-52425, Jülich, Germany.
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