Document Detail


Quantitative histochemistry of mucosubstance in tracheal epithelium of the macaque monkey.
MedLine Citation:
PMID:  3819379     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Experimentally applied irritants and chronic respiratory diseases appear to alter the amount and composition of secretory cell product in surface epithelium and submucosal glands of pulmonary airways. Previous methods used to quantify these changes have been very time-consuming or have not measured the same components of the airway wall. The present study describes a rapid, reproducible, and standardized automated method for quantifying secretory products. The tracheas from eight macaque monkeys were fixed with glutaraldehyde-paraformaldehyde, embedded in glycol methacrylate, serially sectioned at 2 microns, and histochemically stained to demonstrate neutral, sialylated, and sulfated mucosubstances in the cartilaginous, intercartilaginous, and membranous regions of both proximal and distal trachea. Volume densities were determined using an image analyzer and are expressed as volume of stained mucosubstance per unit surface area of epithelial basal lamina. Comparison of the automated method to manual point counting and evaluation of internal variance showed that the automated method had a twelve-fold increase in efficiency with no significant differences in measurements. After weighting the values of each region according to their anatomical contribution, the total secretory product (TSP) for the entire trachea was determined. Periodate-reactive acid material predominated (73%) in luminal surface epithelium, and neutral material predominated (78%) in submucosal glands. Surface epithelium contained 66% of the TSP. The greater contribution by surface epithelium and predominance of acid mucins there resulted in a TSP from the trachea that consisted of 59% acid material (most of which was sulfated) and 41% neutral material. The method proved to be a valid, reproducible, and rapid technique for evaluating variability in abundance of mucosubstances within airway epithelium.
Authors:
J G Heidsiek; D M Hyde; C G Plopper; J A St George
Related Documents :
18590529 - A rapid and robust method for simultaneously measuring changes in the phytohormones aba...
12817459 - Comparisons of amino acid availability by different methods and metabolizable energy de...
8909789 - 13c nmr spectroscopy and gas chromatograph--combustion--isotope ratio mass spectrometry...
1621959 - A colorimetric method for the quantitation of uronic acids and a specific assay for gal...
18961029 - Determination of phosphorus in coke by the oxygen-flask method.
6789289 - A simple routine method for mono and diamine oxidase estimation in human serum.
12126449 - Rearrangement of epoxynitriles: a convenient homologation of acyclic and cyclic ketones...
7695069 - Atopic asthma caused by candida albicans acid protease: case reports.
21212559 - Tigloylshikonin, a new minor shikonin derivative, from the roots and the commercial roo...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  35     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  1987 Apr 
Date Detail:
Created Date:  1987-04-16     Completed Date:  1987-04-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  435-42     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cartilage / analysis
Epithelium / analysis
Exocrine Glands / analysis*
Female
Histocytochemistry / methods
Macaca mulatta
Macaca radiata
Male
Mucus / analysis*
Trachea / analysis*
Grant Support
ID/Acronym/Agency:
HL28978/HL/NHLBI NIH HHS; P01-ES00628/ES/NIEHS NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Rapid-freezing and malachite green-acrolein-osmium tetroxide freeze-substitution fixation improve vi...
Next Document:  Pressor response to NaCl solution administered intracerebroventricularly or intracisternally to cons...