Document Detail

Quantitative histochemical investigations of semipermeable membrane techniques for the assay of acid phosphatase in skeletal muscle. III. A modified simultaneous coupling technique.
MedLine Citation:
PMID:  7263298     Owner:  NLM     Status:  MEDLINE    
Meijer's semipermeable membrane technique for acid phosphatase was modified by treating sections immediately after incubation with 70% ethanol for 30 min at room temperature instead of with formalin. The modification improved the validity of the technique considerably, by stabilising the specific final reaction product formed in presumed enzyme-containing sites. The modified technique thus seems promising for assaying the activity of acid phosphatase in sections of skeletal muscle.
P J Stoward; B Al-Sarraj
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Histochemistry     Volume:  71     ISSN:  0301-5564     ISO Abbreviation:  Histochemistry     Publication Date:  1981  
Date Detail:
Created Date:  1981-10-14     Completed Date:  1981-10-14     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0411300     Medline TA:  Histochemistry     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  599-607     Citation Subset:  IM    
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MeSH Terms
Acid Phosphatase / metabolism*
Muscles / enzymology*
Time Factors
Reg. No./Substance:
50-00-0/Formaldehyde; 64-17-5/Ethanol; EC Phosphatase

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