Document Detail

Quantitative high-performance liquid chromatography analysis of the pool levels of undecaprenyl phosphate and its derivatives in bacterial membranes.
MedLine Citation:
PMID:  19110475     Owner:  NLM     Status:  MEDLINE    
Undecaprenyl phosphate is the essential lipid involved in the transport of hydrophilic motifs across the bacterial membranes during the synthesis of cell wall polymers such as peptidoglycan. A HPLC procedure was developed for the quantification of undecaprenyl phosphate and its two derivatives, undecaprenyl pyrophosphate and undecaprenol. During the exponential growth phase, the pools of undecaprenyl phosphate and undecaprenyl pyrophosphate were ca. 75 and 270 nmol/g of cell dry weight, respectively, in Escherichia coli, and ca. 50 and 150 nmol/g, respectively, in Staphylococcus aureus. Undecaprenol was detected in S. aureus (70 nmol/g), but not in E. coli (<1 nmol/g).
Hélène Barreteau; Sophie Magnet; Meriem El Ghachi; Thierry Touzé; Michel Arthur; Dominique Mengin-Lecreulx; Didier Blanot
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-12-11
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  877     ISSN:  1570-0232     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2009-01-12     Completed Date:  2009-04-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  213-20     Citation Subset:  IM    
Université Paris-Sud, UMR 8619, Institut de Biochimie et Biophysique Moléculaire et Cellulaire, 91405 Orsay Cedex, France.
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MeSH Terms
Cell Membrane / chemistry*
Chromatography, High Pressure Liquid
Escherichia coli / chemistry*
Membrane Lipids / chemistry
Polyisoprenyl Phosphates / analysis*,  metabolism*
Staphylococcus aureus / chemistry*
Terpenes / analysis
Reg. No./Substance:
0/Membrane Lipids; 0/Polyisoprenyl Phosphates; 0/Terpenes; 15575-14-1/undecaprenol; 25126-51-6/undecaprenyl phosphate; 31867-59-1/undecaprenyl pyrophosphate

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