Document Detail


Quantitative footprinting analysis of the chromomycin A3--DNA interaction.
MedLine Citation:
PMID:  1390717     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chromomycin A3 (CHR) binding to the duplex d(CAAGTCTGGCCATCAGTC).d(GACTGATGGCCAGACTTG) has been studied using quantitative footprinting methods. Previous NMR studies indicated CHR binds as a dimer in the minor groove. Analysis of autoradiographic spot intensities derived from DNase I cleavage of the 18-mer in the presence of various amounts of CHR revealed that the drug binds as a dimer to the sequence 5'-TGGCCA-3',3'-ACCGGT-5' in the 18-mer with a binding constant of (2.7 +/- 1.4) x 10(7) M-1. Footprinting and fluorescence data indicate that the dimerization constant for the drug in solution is approximately 10(5) M-1. Since it has been suggested that CHR binding alters DNA to the A configuration, quantitative footprinting studies using dimethyl sulfate, which alkylates at N-7 of guanine in the major groove, were also carried out. Apparently, any drug-induced alteration in DNA structure does not affect cleavage by DMS enough to be observed by these experiments.
Authors:
A Stankus; J Goodisman; J C Dabrowiak
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemistry     Volume:  31     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  1992 Sep 
Date Detail:
Created Date:  1992-11-13     Completed Date:  1992-11-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  9310-8     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Syracuse University, New York 13244-4100.
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MeSH Terms
Descriptor/Qualifier:
Base Composition
Base Sequence
Binding Sites
Chromomycin A3 / chemistry*
DNA / chemistry*
Indicators and Reagents
Kinetics
Mathematics
Molecular Sequence Data
Oligodeoxyribonucleotides / chemistry*
Chemical
Reg. No./Substance:
0/Indicators and Reagents; 0/Oligodeoxyribonucleotides; 7059-24-7/Chromomycin A3; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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