| Quantitative fluorescence in situ hybridization (QFISH) of telomere lengths in tissue and cells. | |
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MedLine Citation:
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PMID: 18770817 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Telomeres are repetitive DNA sequences at the end of each chromosome that provide stability and prevent end-to-end chromosome fusions. In order to understand mechanisms responsible for telomere shortening, it is necessary to develop methods for accurate telomere length measurement that can be applied to archival and fresh tissue and cells. This unit describes in situ-based quantitative fluorescence in situ hybridization (QFISH) protocols using a fluorescence-conjugated telomere probe (peptide nucleic acid, PNA) that stains telomeres proportionally to their length. These protocols can be used on formalin-fixed paraffin-embedded tissue, lightly fixed tissue, cells isolated from tissue, cultured cells, and agar-embedded cells. The basic protocol for QFISH staining is modified to achieve excellent QFISH staining for a variety of cell preparations. Image-analysis techniques to quantitate average telomere lengths from tissues and isolated stained cells are also described. |
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Authors:
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Jacintha N O'Sullivan; Jennifer C Finley; Rosa-Ana Risques; Wen-Tang Shen; Katherine A Gollahon; Peter S Rabinovitch |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.] Volume: Chapter 12 ISSN: 1934-9300 ISO Abbreviation: Curr Protoc Cytom Publication Date: 2005 Aug |
Date Detail:
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Created Date: 2008-09-04 Completed Date: 2008-10-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100899351 Medline TA: Curr Protoc Cytom Country: United States |
Other Details:
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Languages: eng Pagination: Unit 12.6 Citation Subset: IM |
Affiliation:
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St Vincent's University Hospital, Elm Park, Dublin, Ireland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells / cytology, ultrastructure Chromosomes / physiology, ultrastructure Formaldehyde Image Processing, Computer-Assisted In Situ Hybridization / methods* Spectrometry, Fluorescence / methods Telomere / ultrastructure* |
| Chemical | |
Reg. No./Substance:
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50-00-0/Formaldehyde |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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