Document Detail

Quantitative evaluation of bacteria washed from stainless steel penicylinders during AOAC use-dilution method.
MedLine Citation:
PMID:  3148609     Owner:  NLM     Status:  MEDLINE    
Stainless steel penicylinders inoculated separately with test bacteria (Salmonella choleraesuis, Pseudomonas aeruginosa, or Staphylococcus aureus) are used in the AOAC use-dilution method (UDM) for disinfectant efficacy testing. Numbers of bacteria remaining on penicylinders were quantitatively assessed to determine if cells are washed from the penicylinders after a 10 min exposure to phosphate buffer dilution water (PBDW). Inoculated penicylinders were also examined by scanning electron microscopy (SEM) to determine the presence of cells remaining attached to the penicylinders after a 10 min exposure to a quaternary ammonium disinfectant and separately to PBDW. The percentage of cells washed from inoculated penicylinders exposed to PBDW was 89.9 for Salmonella choleraesuis, 48.8 for Pseudomonas aeruginosa, and 38.8 for Staphylococcus aureus. Qualitative examination of penicylinders by scanning electron microscopy confirmed the attachment of S. aureus and P. aeruginosa cells to penicylinders exposed separately to PBDW and a quaternary ammonium disinfectant. Few S. choleraesuis cells were observed on penicylinders exposed to PBDW and no cells were observed after disinfectant exposure. The variability of the numbers of viable cells entering the recovery media among the 3 UDM test bacteria due to cell detachment could be a significant factor in the recognized variability of the use-dilution method.
E M Alfano; E C Cole; W A Rutala
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal - Association of Official Analytical Chemists     Volume:  71     ISSN:  0004-5756     ISO Abbreviation:  J Assoc Off Anal Chem     Publication Date:    1988 Sep-Oct
Date Detail:
Created Date:  1989-04-28     Completed Date:  1989-04-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7505559     Medline TA:  J Assoc Off Anal Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  868-71     Citation Subset:  IM    
University of North Carolina, Department of Medicine, Chapel Hill 27599.
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MeSH Terms
Bacteriological Techniques / instrumentation*
Culture Media
Microscopy, Electron, Scanning
Pseudomonas aeruginosa / isolation & purification
Salmonella / isolation & purification
Staphylococcus aureus / isolation & purification
Reg. No./Substance:
0/Culture Media

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