Document Detail


Quantitative energy-filtered image analysis in cytochemistry. I. Morphometric analysis of contrast-related images.
MedLine Citation:
PMID:  2336734     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A combination of energy-filtered electron microscopy (EFEM) and an image-analyzing system (IBAS/2000) is used for morphometric analyses of cells and (reaction) products. Image contrast is objectively established and segmentation is based upon intrinsic contrasts, in ultrathin sections. Cross-sectioned platinum-stained erythrocytes are used as a model to determine optimal conditions for constant measuring results for contrast, area and perimeter. Results are related to changes in: (1) the objective-lens diaphragm diameter, (2) three most frequently used contrast modes obtainable by electron spectroscopical imaging (ESI) in a Zeiss EM 902 transmission electron microscope (e.g., global, zero loss (or deltaE - 0 eV) and deltaE = 250 eV), and (3) the number of image integrations (1-250X) acquired by real-time video. A thresholding procedure is proposed for objective segmentation of such contrast-related images and applied to measure the area fraction of nuclear chromatin and the diameter of nominal 1 nm colloidal gold particles.
Authors:
C W Sorber; A A De Jong; N J Den Breejen; W C De Bruijn
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Ultramicroscopy     Volume:  32     ISSN:  0304-3991     ISO Abbreviation:  Ultramicroscopy     Publication Date:  1990 Jan 
Date Detail:
Created Date:  1990-06-12     Completed Date:  1990-06-12     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7513702     Medline TA:  Ultramicroscopy     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  55-68     Citation Subset:  IM    
Affiliation:
AEM Unit, Clinical Pathological Institute I, Erasmus University Rotterdam, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line, Transformed
Cells, Cultured
Erythrocytes / ultrastructure*
Histocytochemistry
Image Processing, Computer-Assisted
Macrophages
Mice
Microscopy, Electron

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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