Document Detail

Quantitative energy-filtered image analysis in cytochemistry. I. Morphometric analysis of contrast-related images.
MedLine Citation:
PMID:  2336734     Owner:  NLM     Status:  MEDLINE    
A combination of energy-filtered electron microscopy (EFEM) and an image-analyzing system (IBAS/2000) is used for morphometric analyses of cells and (reaction) products. Image contrast is objectively established and segmentation is based upon intrinsic contrasts, in ultrathin sections. Cross-sectioned platinum-stained erythrocytes are used as a model to determine optimal conditions for constant measuring results for contrast, area and perimeter. Results are related to changes in: (1) the objective-lens diaphragm diameter, (2) three most frequently used contrast modes obtainable by electron spectroscopical imaging (ESI) in a Zeiss EM 902 transmission electron microscope (e.g., global, zero loss (or deltaE - 0 eV) and deltaE = 250 eV), and (3) the number of image integrations (1-250X) acquired by real-time video. A thresholding procedure is proposed for objective segmentation of such contrast-related images and applied to measure the area fraction of nuclear chromatin and the diameter of nominal 1 nm colloidal gold particles.
C W Sorber; A A De Jong; N J Den Breejen; W C De Bruijn
Related Documents :
15834124 - Lipid imaging by gold cluster time-of-flight secondary ion mass spectrometry: applicati...
8760404 - Quantitative electron spectroscopic imaging in bio-medicine: evaluation and application.
10872044 - Nuclear volume estimation using different sampling, measurement and calculation methods.
3277924 - Quantitative digital subtraction coronary angiography using videodensitometry. an in vi...
24791614 - The imaging spectrum of bronchopulmonary sequestration.
14709684 - Ultrasonographic modalities to assess vascular anatomy and disease.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Ultramicroscopy     Volume:  32     ISSN:  0304-3991     ISO Abbreviation:  Ultramicroscopy     Publication Date:  1990 Jan 
Date Detail:
Created Date:  1990-06-12     Completed Date:  1990-06-12     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7513702     Medline TA:  Ultramicroscopy     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  55-68     Citation Subset:  IM    
AEM Unit, Clinical Pathological Institute I, Erasmus University Rotterdam, The Netherlands.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Line, Transformed
Cells, Cultured
Erythrocytes / ultrastructure*
Image Processing, Computer-Assisted
Microscopy, Electron

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Computerized three-dimensional reconstructions of serial sections in electron microscopy.
Next Document:  Quantitative energy-filtered image analysis in cytochemistry. II. Morphometric analysis of element-d...