| Quantitative and dynamic assay of single cell chemotaxis. | |
| | |
MedLine Citation:
|
PMID: 22230969 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
We have developed a single-cell assay platform that allows quantitative analysis of single cell chemotaxis by dynamic morphogenetic gradients, subcellular microscopic imaging and automated image analysis, and have applied these to measure cellular polarization of budding yeast. The computer-controlled microfluidic device regulates the gradient profile at any given time, and allows quantitative monitoring of cell morphology and the localization and expression of specific marker proteins during the dynamic polarization process. With this integrated experimental system, we compare the polarized signaling response of wild-type and far1-H7 mutant cells, which express a truncated Far1 protein unable to interact with Cdc24. Our results confirm that Far1 functions as an adaptor that recruits polarity establishment proteins to the site of extracellular signaling. Moreover, by changing the gradient profile and estimating the number of bound surface receptors, we quantitatively address why surprisingly small differences in pheromone concentration across yeast cells can be amplified into a robust polarity axis. This integrated single cell experimental platform thus opens the possibility to quantitatively investigate the molecular regulatory mechanism of chemotaxis in yeast, which serves as a paradigm to understand the fundamental processes involved in cancer metastasis, angiogenesis and axon generation. |
| | |
Authors:
|
Sung Sik Lee; Peter Horvath; Serge Pelet; Björn Hegemann; Luke P Lee; Matthias Peter |
Related Documents
:
|
21984949 - Anti-aβ drug screening platform using human ips cell-derived neurons for the treatment... 21673499 - The role of rae1 in nup98 fusion-mediated leukemogenesis. 21572589 - Discovery of 3,5-diamino-1,2,4-triazole ureas as potent anaplastic lymphoma kinase inhi... 22801379 - Endocrine cells and nerve ganglia of the small intestine of the opossum didelphis aurit... 21821139 - Moving in the right direction: how eukaryotic cells migrate along chemical gradients. 21704599 - Novel nanostructured lipid-dextran sulfate hybrid carriers (nldcs) overcome tumor multi... 21558269 - Plasma membrane aquaporin aqpz protein is essential for glucose metabolism during photo... 12040649 - Fine needle aspiration cytology of fibroadenoma with multinucleated stromal giant cells... 84489 - Measurement and differentiation of the cellular infiltrate in expermiental allergic con... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-1-9 |
Journal Detail:
|
Title: Integrative biology : quantitative biosciences from nano to macro Volume: - ISSN: 1757-9708 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
|
Created Date: 2012-1-10 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101478378 Medline TA: Integr Biol (Camb) Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Institute of Biochemistry, ETH Zurich, Zurich, CH 8093, Switzerland. matthias.peter@bc.biol.ethz.ch. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A Cre-Lox P Recombination Approach for the Detection of Cell Fusion <em>In Vivo</em>.
Next Document: Review on the application of nanobiosensors in food analysis.