Document Detail


Quantitative double antibody sandwich ELISA for the determination of gliadin.
MedLine Citation:
PMID:  22963484     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
A sensitive double antibody sandwich ELISA (DAS-ELISA) based on chicken anti-gliadin IgY and biotinylated monoclonal antibody (mAb) was developed for the quantification of gliadin in foods. The anti-gliadin IgY and mAb specifically detected gliadin in wheat, barley, and rye by indirect ELISA and Western-blot assay. Using anti-gliadin IgY as capture antibody and biotinylated mAb as detecting antibody, the sensitivity of DAS-ELISA has a linear standard range of 4-40 ng/mL, showing that the limit of detection (LOD) corresponds to 4 ng/mL gliadin in assay buffer, equivalent to 0.8 ppm in foods. The intra-assay expressed as percentage of coefficients of variation (%CV) was 7.25% average of six food samples. The interassay precision was 9.51% in food samples. The combination of anti-gliadin IgY and biotinylated mAb in the DAS-ELISA provides a reliable, sensitive, and inexpensive tool for the detection of gliadin in gluten-free and gluten-containing food products.
Authors:
Naiyana Gujral; Mavanur R Suresh; Hoon H Sunwoo
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of immunoassay & immunochemistry     Volume:  33     ISSN:  1532-4230     ISO Abbreviation:  J Immunoassay Immunochem     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100963688     Medline TA:  J Immunoassay Immunochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  339-51     Citation Subset:  IM    
Affiliation:
a Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta , Edmonton , Alberta , Canada.
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