| Quantitative differences in Ia antigen expression in the spleens of 15I5-B congenic and inbred chickens as defined by a new monoclonal antibody. | |
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MedLine Citation:
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PMID: 2467275 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A monoclonal antibody (MAb), designated P2M11, that detects a monomorphic determinant of chicken class II antigens was produced from the fusion of P3X63 myeloma cells with spleen cells from BALB/c mice immunized with chicken splenic lymphocytes. Flow cytometric analyses of lymphocytes from the SC and FP strains of chickens showed 30 to 50% staining of bursa cells, 15 to 20% staining cells, and less than 5% staining of thymus cells. Addition of MAb P2M11 to splenic of T cell cultures stimulated with allogeneic cells or concanavalin A resulted in a significant inhibition of the T cell proliferation responses. Immunoprecipitation of 35S-methionine-labeled spleen cell extracts using MAb P2M11 identified molecules with apparent molecular weights of approximately 28,000, 30,000, and 32,000 by sodium dodecyl-polyacryl-amide gel electrophoresis. Taken together, these data indicate that the antigens detected by MAb P2M11 are similar in cell distribution and structure to chicken Ia antigens encoded by B-L genes. Using this MAb, a strain difference was demonstrated in the tissue distribution of Ia antigen positive lymphocytes in the spleens but not the thymuses of 15I5-B congenic and inbred strains of chickens. This difference may be due to the genes associated with B-complex genes. |
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Authors:
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H S Lillehoj; S Kim; E P Lillehoj; L D Bacon |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Poultry science Volume: 67 ISSN: 0032-5791 ISO Abbreviation: Poult. Sci. Publication Date: 1988 Nov |
Date Detail:
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Created Date: 1989-04-24 Completed Date: 1989-04-24 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0401150 Medline TA: Poult Sci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1525-35 Citation Subset: IM |
Affiliation:
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USDA, Protozoan Diseases Laboratory, Beltsville, Maryland 20705. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal / immunology* Chickens / immunology* Epitopes / analysis, genetics* Histocompatibility Antigens Class II / analysis, genetics* Lymphocytes / immunology* Spleen / immunology* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Epitopes; 0/Histocompatibility Antigens Class II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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