| Quantitative determination of sulfated glycopeptide by two-dimensional electrophoresis on cellulose acetate membrane. | |
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MedLine Citation:
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PMID: 2809069 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Quantitative determination of the sulfated glycoproteins present in tissue and secretion fluid was performed. After digestion of the specimen with pronase in order to convert glycoproteins to glycopeptides, the sulfated glycopeptides were separated from a mixture of acidic glycans (glycosaminoglycans, sialoglycopeptides and sulfated glycopeptides) by two-dimensional electrophoresis on cellulose acetate membrane [(1986) J. Biochem. Biophys. Methods 12, 239-246]. After staining with alcian blue, the spot of sulfated glycopeptide on the cellulose acetate membrane was cut out, and then only the dye bound to the sulfated glycopeptide was extracted with a 5% cetylpyridinium chloride solution at 100 degrees C for 15 min. The extract was then measured by absorbance at 615 nm using an authentic sulfated glycopeptide as a standard. This method facilitated the determination of sulfated glycopeptides, which were separated from other acidic glycans, within the range 0-25 micrograms. |
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Authors:
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K Muramoto; H Tanaka; S Kimura; K Kubo; S Yoshihara; M Yokoyama; Y Yoshida; M Endo |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of biochemical and biophysical methods Volume: 19 ISSN: 0165-022X ISO Abbreviation: J. Biochem. Biophys. Methods Publication Date: 1989 Jul |
Date Detail:
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Created Date: 1989-12-12 Completed Date: 1989-12-12 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 7907378 Medline TA: J Biochem Biophys Methods Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 75-82 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Hirosaki University School of Medicine, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbohydrates / analysis Clusterin Electrophoresis, Cellulose Acetate / methods Electrophoresis, Gel, Two-Dimensional / methods Glycoproteins* Glycosaminoglycans* Indicators and Reagents Intestinal Mucosa / analysis Intestine, Small / analysis Molecular Chaperones* Pronase Swine |
| Chemical | |
Reg. No./Substance:
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0/Carbohydrates; 0/Clusterin; 0/Glycoproteins; 0/Glycosaminoglycans; 0/Indicators and Reagents; 0/Molecular Chaperones; EC 3.4.24.-/Pronase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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