| Quantitative determination of peptide drug in human plasma samples at low pg/ml levels using coupled column liquid chromatography-tandem mass spectrometry. | |
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MedLine Citation:
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PMID: 20456896 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Plasma concentrations after administration of peptide drugs are often low due to the high potency often seen with this class of compounds. In this work a bioanalytical method based on coupled column liquid chromatography-tandem mass spectrometry (LC-MS/MS) is presented for quantification of a peptide drug, FE 202158, under clinical development. A volume of 0.5 ml human plasma is solid phase extracted on a weak cationic exchanger. After evaporation of the solvent to dryness, the reconstituted sample is injected into a coupled column liquid chromatography system. A heart-cut from the initial column, a cyano column, is trapped on a C(4) column and thereafter injected into a microbore C(18) column. For the detection a triple quadrupole mass spectrometer, equipped with a TurboIonSpray interface working in positive ion mode, is used. The design of the system is described and the gain in sensitivity and selectivity, compared to a conventional system, is discussed. Data from validation of the bioanalytical method are presented. For human plasma samples a lower limit of quantification (LLOQ) of 5.00 pg/ml (=4.77 pmol/l) was achieved. The inter-assay precision was less than 11% and bias was within +/-4% over the whole validated range of 5.00-860 pg/ml. |
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Authors:
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Ulf Lövgren; Sara Johansson; Lasse Skov Jensen; Carina Ekström; Alf Carlshaf |
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Publication Detail:
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Type: Journal Article Date: 2010-03-27 |
Journal Detail:
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Title: Journal of pharmaceutical and biomedical analysis Volume: 53 ISSN: 1873-264X ISO Abbreviation: J Pharm Biomed Anal Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-07-20 Completed Date: 2010-09-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8309336 Medline TA: J Pharm Biomed Anal Country: England |
Other Details:
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Languages: eng Pagination: 537-45 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Bioanalysis, Experimental Medicine & Bioanalysis, Clinical & Non-Clinical R & D, Ferring Pharmaceuticals A/S, Kay Fiskers Plads 11, 2300 Copenhagen S, Denmark. ulf.lovgren@ferring.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, Liquid
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methods* Humans Peptides / blood* Pharmaceutical Preparations / blood* Sensitivity and Specificity Solid Phase Extraction Tandem Mass Spectrometry / methods* |
| Chemical | |
Reg. No./Substance:
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0/Peptides; 0/Pharmaceutical Preparations |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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