Document Detail

Quantitative determination of hydroxycinnamic acids in wheat, rice, rye, and barley straws, maize stems, oil palm frond fiber, and fast-growing poplar wood.
MedLine Citation:
PMID:  11714291     Owner:  NLM     Status:  MEDLINE    
A new method has been developed for the quantitative determination of hydroxycinnamic acids participating in ester or ether linkages to the cell wall polymers. The method is based on mild alkaline hydrolysis followed by acid hydrolysis or mild alkaline hydrolysis, which partially removed esterified phenolic acids, and high-temperature concentrated alkaline treatment, which cleaved both the ester and ether linkages. It was found that traditional mild alkaline hydrolysis and acid hydrolysis released only part of the ester- and ether-linked phenolic acids, respectively. Approximately half (44.0-47.9%) of the total ester-linked p-coumaric acid and 18.2-32.6% of the total esterified ferulic acid remained ester-linked to the mild alkali-soluble lignin polymers, and 55.0-72.0% of the total ether-linked p-coumaric acid and 37.5-53.8% of the total ether-linked ferulic acid remained ether-linked to the solubilized lignin molecules after the acid hydrolysis. To correct this, a second mild alkaline hydrolysis of the alkali-soluble lignin preparations and acid hydrolysis of the solubilized lignin fractions, obtained from the first acid hydrolysis of the cell wall materials, was investigated. On the basis of this new method, a majority of the cell wall p-coumaric acid (55.8-81.5%) was found to be ester-linked to cell wall components, mainly to lignin, and about half of the cell wall ferulic acid is etherified through its phenolic oxygen to the cell wall lignin component, whereas the remainder is esterified to the cell wall hemicelluloses and/or lignin in different plant materials.
R C Sun; X F Sun; S H Zhang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  49     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-11-20     Completed Date:  2002-01-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5122-9     Citation Subset:  IM    
State Key Laboratory of Pulp and Paper Engineering, College of Paper and Environment Engineering, South China University of Technology, Guangzhou 510640, People's Republic of China.
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MeSH Terms
Cereals / chemistry*
Chromatography, High Pressure Liquid
Coumaric Acids / analysis*
Magnetic Resonance Spectroscopy
Spectroscopy, Fourier Transform Infrared
Trees / chemistry*
Reg. No./Substance:
0/Coumaric Acids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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