Document Detail

Quantitative determination of free and hydrolytically liberated beta-damascenone in red grapes and wines using a stable isotope dilution assay.
MedLine Citation:
PMID:  10444847     Owner:  NLM     Status:  MEDLINE    
Quantitative analysis of free and hydrolytically liberated beta-damascenone in grapes and wines was developed, using a stable isotope dilution assay. Free beta-damascenone was isolated from grapes and wines by diethyl ether-hexane (1:1, v/v) extraction and the precursor(s) (glycosidic, polyols) of beta-damascenone using Sep-Pak Plus C18 RP cartridges. Hydrolytically liberated beta-damascenone was generated by acid hydrolysis from the precursor(s) extract. Red wines from Bordeaux (Merlot, Cabernet Sauvignon and Cabernet Franc, 1995 and 1996 vintage), Burgundy (Pinot Noir, 1995 and 1996 vintage) regions and Grenache wines from Chateauneuf du Pape and Côtes du Rhône (1995 vintage) were analysed to quantify free beta-damascenone. The wines made from Grenache and Cabernet Sauvignon (1996 vintage) grapes presented the highest mean amounts of free beta-damascenone, 5.4 and 5.5 micrograms l-1, respectively. Merlot, Cabernet Sauvignon and Cabernet Franc grapes of Bordeaux (1996 vintage) and their corresponding wines were analysed for quantification of free and hydrolytically liberated beta-damascenone. The levels of hydrolytically liberated beta-damascenone in grapes could predict closely the levels of free beta-damascenone in the corresponding wines after one year of ageing, i.e., almost half the levels found for the grape samples. The influence of enzyme and heat treatment of Merlot wine samples on their beta-damascenone levels was studied. Heat treatment doubled the levels of this compound, but enzyme treatment generated, in the corresponding wines, half the levels of beta-damascenone found in the non-enzyme treated wines.
Y Kotseridis; R L Baumes; G K Skouroumounis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography. A     Volume:  849     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  1999 Jul 
Date Detail:
Created Date:  1999-08-24     Completed Date:  1999-08-24     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  245-54     Citation Subset:  IM    
Laboratoire des Arômes et Substances Naturelles, INRA-IPV, Montpellier, France.
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MeSH Terms
Gas Chromatography-Mass Spectrometry
Reproducibility of Results
Rosales / chemistry*
Sensitivity and Specificity
Terpenes / analysis*
Wine / analysis*
Reg. No./Substance:
0/Isotopes; 0/Norisoprenoids; 0/Terpenes; 23696-85-7/beta-damascenone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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