Document Detail

Quantitative determination of factor-VIII protein by two-stage gel electrophoresis.
MedLine Citation:
PMID:  1084158     Owner:  NLM     Status:  MEDLINE    
A new method for the quantification of factor-VIII protein in plasma is described which utilizes only commercially available equipment and material. The procedure involves separation of factor VII from other plasma proteins by a two-stage sodium dodecyl sulphate gel electrophoretic technique and quantification of the protein by scanning densitometry. The plasma from normal individuals, haemophiliacs, obligate carriers of haemophilia and patients with von Willebrand's disease have been examined by this technique. In general, the data support the earlier findings obtained by quantitative immunoelectrophoresis. In addition, some abnormalities not detected by immunoelectrophoresis are observed by the gel electrophoresis technique.
M J Weinstein; D Deykin; E W Davie
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  British journal of haematology     Volume:  33     ISSN:  0007-1048     ISO Abbreviation:  Br. J. Haematol.     Publication Date:  1976 Jul 
Date Detail:
Created Date:  1976-09-02     Completed Date:  1976-09-02     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372544     Medline TA:  Br J Haematol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  343-55     Citation Subset:  IM    
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MeSH Terms
Electrophoresis, Agar Gel / methods
Electrophoresis, Polyacrylamide Gel / methods
Factor VIII / analysis*
Hemophilia A / blood,  genetics
Time Factors
von Willebrand Diseases / blood
Reg. No./Substance:
9001-27-8/Factor VIII

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