| Quantitative determination of apoptosis on leukemia cells by infrared spectroscopy. | |
| | |
MedLine Citation:
|
PMID: 11445669 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Quantitation of apoptotic cell death in vivo has become an important issue for patients with acute leukemia. We describe herein a new analytical method, based on infrared (IR) spectroscopy, to estimate the percentage of apoptotic leukemic cells in two different cell lines (CEM and K562), induced with etoposide (VP-16). As the percentage of apoptosis increases, the protein structure shifts from dominantly beta-sheet to unordered (random coil), the overall lipid content increases and the amount of detectable DNA decreases. These changes can be directly related to the percentage of apoptosis as determined by two standard reference methods: flow cytometry and DNA ladder formation. The correlation between the significant IR spectral changes and the percentage of apoptotic leukemia cells in the two cell lines was optimal up to 24 h following etoposide treatment (r = 0.99 for CEM cells and r = 0.96 for K562 cells). Furthermore, IR spectroscopy is able to detect apoptotic changes in these cells already after 4 h treatment with VP-16, compared to flow cytometry which needs 6 h to observe significant changes. Our study suggests that IR spectroscopy may have potential clinical utility for the early, fast and reagent free assessment of chemotherapeutic efficacy in patients with leukemia. |
| | |
Authors:
|
K Z Liu; L Jia; S M Kelsey; A C Newland; H H Mantsch |
Related Documents
:
|
20840769 - Antiproliferative effect of tualang honey on oral squamous cell carcinoma and osteosarc... 10543259 - Retention of cell adhesion and growth capability in human cervical cancer cells deprive... 16609959 - Cannabinoid agonist win55,212-2 induces apoptosis in cerebellar granule cells via activ... 19041749 - Motor deficit in a drosophila model of mucolipidosis type iv due to defective clearance... 15455089 - Effects of phosphoinositide cycle modifiers on apoptosis of peritoneal macrophages. 20405239 - Protective effect of pacap against doxorubicin-induced cell death in cardiomyocyte cult... 21688189 - Ciliogenesis in normal human kidney development and post-natal life. 6300179 - Characterization of the synthesis and release of prolactin by an enriched fraction of h... 15910749 - Deferoxamine photosensitizes cancer cells in vitro. |
Publication Detail:
|
Type: Evaluation Studies; Journal Article |
Journal Detail:
|
Title: Apoptosis : an international journal on programmed cell death Volume: 6 ISSN: 1360-8185 ISO Abbreviation: Apoptosis Publication Date: 2001 Aug |
Date Detail:
|
Created Date: 2001-07-10 Completed Date: 2001-11-01 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9712129 Medline TA: Apoptosis Country: United States |
Other Details:
|
Languages: eng Pagination: 269-78 Citation Subset: IM |
Affiliation:
|
Institute for Biodiagnostics, National Research Council of Canada, Winnipeg, MB, Canada. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Apoptosis* Cell Count Etoposide / pharmacology Flow Cytometry Leukemia / pathology* Spectrophotometry, Infrared* Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
|
33419-42-0/Etoposide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Phosphatidylserine induces apoptosis in adherent cells.
Next Document: Progressively developed myocardial apoptotic cell death during late phase of reperfusion.