Document Detail

Quantitative determination of apoptosis on leukemia cells by infrared spectroscopy.
MedLine Citation:
PMID:  11445669     Owner:  NLM     Status:  MEDLINE    
Quantitation of apoptotic cell death in vivo has become an important issue for patients with acute leukemia. We describe herein a new analytical method, based on infrared (IR) spectroscopy, to estimate the percentage of apoptotic leukemic cells in two different cell lines (CEM and K562), induced with etoposide (VP-16). As the percentage of apoptosis increases, the protein structure shifts from dominantly beta-sheet to unordered (random coil), the overall lipid content increases and the amount of detectable DNA decreases. These changes can be directly related to the percentage of apoptosis as determined by two standard reference methods: flow cytometry and DNA ladder formation. The correlation between the significant IR spectral changes and the percentage of apoptotic leukemia cells in the two cell lines was optimal up to 24 h following etoposide treatment (r = 0.99 for CEM cells and r = 0.96 for K562 cells). Furthermore, IR spectroscopy is able to detect apoptotic changes in these cells already after 4 h treatment with VP-16, compared to flow cytometry which needs 6 h to observe significant changes. Our study suggests that IR spectroscopy may have potential clinical utility for the early, fast and reagent free assessment of chemotherapeutic efficacy in patients with leukemia.
K Z Liu; L Jia; S M Kelsey; A C Newland; H H Mantsch
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Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Apoptosis : an international journal on programmed cell death     Volume:  6     ISSN:  1360-8185     ISO Abbreviation:  Apoptosis     Publication Date:  2001 Aug 
Date Detail:
Created Date:  2001-07-10     Completed Date:  2001-11-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9712129     Medline TA:  Apoptosis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  269-78     Citation Subset:  IM    
Institute for Biodiagnostics, National Research Council of Canada, Winnipeg, MB, Canada.
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MeSH Terms
Cell Count
Etoposide / pharmacology
Flow Cytometry
Leukemia / pathology*
Spectrophotometry, Infrared*
Tumor Cells, Cultured
Reg. No./Substance:

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