Document Detail

Quantitative determination of amino acids in functional foods by microchip electrophoresis.
MedLine Citation:
PMID:  18266297     Owner:  NLM     Status:  MEDLINE    
Microchip electrophoresis (MCE), a first-generation micrototal analysis system, has emerged during the miniaturization phase of food analysis. Based on the micellar electrokinetic chromatography mode, a simple and fast MCE method with light emitting diode-induced fluorescence detection was developed for quantitative analysis of amino acids in three different kinds of functional foods, viz. sports beverages, jelly-form beverages, and tablet-form functional foods. In contrast to the glass microchip, we improved the separation of amino acids on a poly(methyl methacrylate) (PMMA) chip by addition of cationic starch derivatives. 4-fluoro-7-nitro-2,1,3-benzoxadiazole, which has a short labeling time for amino acids, was used as the fluorescently labeled dye. This MCE method takes less than 10 min of total analysis time including sample preparation and analysis of amino acids in functional foods on a PMMA chip. The results show that this approach has the potential to be a fast and simple method for amino acid analysis in functional foods.
Hiroko Ueno; Jun Wang; Noritada Kaji; Manabu Tokeshi; Yoshinobu Baba
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Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Journal of separation science     Volume:  31     ISSN:  1615-9314     ISO Abbreviation:  J Sep Sci     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-03-24     Completed Date:  2008-06-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101088554     Medline TA:  J Sep Sci     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  898-903     Citation Subset:  IM    
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Nagoya, Japan.
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MeSH Terms
Amino Acids / analysis*,  chemistry
Chromatography, Micellar Electrokinetic Capillary / methods*
Electrophoresis, Microchip / methods*
Food Analysis / instrumentation,  methods
Glass / chemistry
Health Food / analysis*
Polymethyl Methacrylate / chemistry
Time Factors
Reg. No./Substance:
0/Amino Acids; 9011-14-7/Polymethyl Methacrylate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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